法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (2): 120-122+.

• 论文 • 上一篇    下一篇

饮料中γ-羟基丁酸的分析

刘伟;沈敏;向平;卜俊;吴何坚;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063,上海200063,上海200063,上海200063,上海200063
  • 发布日期:2007-04-25 出版日期:2007-04-28

Detection of γ-Hydroxybutyrate(GHB) in Beverages

LIU WEI,SHEN MIN,XIANG PING,BU JUN,WU HE-JIAN(INSTITUTE OF FORENSIC SCIENCES,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA)   

  • Online:2007-04-25 Published:2007-04-28

摘要： 目的建立饮料中γ-羟基丁酸(GHB)的分析方法。方法检材以GHB-d6为内标,加入酸性氯化铵饱和溶液调节pH值<4,用乙酸乙酯提取、离心后取有机层,水浴下吹干,经BSTFA衍生化后,用气相色谱/质谱联用仪测定。检材以GHB-d6为内标,经流动相稀释、离心后,吸取上清液用液相色谱-串联质谱仪测定。结果GC/MS测定饮料中GHB的检出限为0.2μg/mL,日内精密度和日间精密度小于8.54%;LC/MS/MS测定饮料中GHB的检出限为2μg/mL,日内精密度和日间精密度小于8.62%。结论饮料中GHB进行定性定量分析。方法灵敏、准确、快速,适用于法庭毒物分析中饮料中GHB的检测。

关键词: γ-羟基丁酸(GHB), 饮料, 气相色谱/质谱联用法, 液相色谱-串联质谱法

Abstract: Objective To establish an analytical method for the determination of GHB in beverages using GC/MS and LC/MS/MS.Methods After beverage samples with GHB-d6 as the internal standard were extracted with ethyl acetate,then the extracts were derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide(BSTFA) ,at last the derivateized extracts analyzed by gas chromatography-mass spectrometry.After beverage samples with GHB-d6 as the internal standard were diluted by mobile phase then directly analyzed by LC/MS/MS.Results The limit of detection was 0.2 μg/mL and both relative standard deviations for between-day and within-day assays were < 8.54% in GC/MS.The limit of detection was 2 μg/mL and both relative standard deviations for between-day and within-day assays were <8.62% in LC/MS/MS.Conclusion These methods of qualitative and quantitative analysis were found to be sensitive,accurate,rapid and suitable for the forensic toxicology to test of GHB in real cases.

Key words: γ-Hydroxybutyrate(GHB), beverage, GC/MS, LC/MS/MS