法医学杂志

法医学杂志 ›› 2008, Vol. 24 ›› Issue (5): 325-326+.

• 论文 • 上一篇    下一篇

皮肤烧伤愈合过程中磷酸化JNK的变化

熊昌艳;官大威;刘志会;甄博;赵锐;朱宝利;   

  1. 中国医科大学法医学院法医病理学教研室;武警辽宁总队医院烧伤科;
  • 发布日期:2008-10-25 出版日期:2008-10-28

Changes of Phospho-JNK Expression during the Skin Burned Wound Healing

XIONG CHANG-YAN1,GUAN DA-WEI1,LIU ZHI-HUI2,ZHEN BO1,ZHAO RUI1,ZHU BAO-LI1 (1. SCHOOL OF FORENSIC MEDICINE,CHINA MEDICAL UNIVERSITY,SHENYANG 110001,CHINA; 2. DEPARTMENT OF BURN,LIAONING PROVINCIAL CORPS HOSPITAL,CHINESE PEOPLE′S ARMED POLICE FORCES,SHENYAN   

  • Online:2008-10-25 Published:2008-10-28

被引次数

5

摘要： 目的观察烧伤后人皮肤磷酸化JNK(p-JNK)的变化特点,初步探讨烧伤后创面愈合的分子机制。方法取12例烧伤后住院植皮患者的烧伤周边区皮肤为烧伤周边皮肤组;另取正常皮肤12例为正常皮肤组。应用免疫组织化学方法和常规HE染色检测p-JNK在皮肤烧伤周边区和正常皮肤组织中的变化情况。结果正常皮肤组织中表皮基底层细胞的胞浆和胞核内有p-JNK阳性着色,阳性细胞率为(8.8±1.3)%。在烧伤周边皮肤组中,p-JNK阳性着色主要定位于表皮细胞和部分炎症细胞中,阳性细胞率上升至(31.2±3.3)%,明显高于正常皮肤组织(P<0.01)。结论烧伤后人皮肤p-JNK的变化可能与创面愈合有关。

关键词: 法医病理学, 烧伤, JNK丝裂原活化蛋白激酶类, 伤口愈合

Abstract: Objective To observe the changes of expression of phosphorylation c-Jun NH2-terminal kinase(p-JNK) during the skin burned wound healing in patients and discuss the molecular mechanism of burned wound healing. Methods The staining intensity and distribution of p-JNK were detected by immunohistochemistry and routine histology in burned skin samples of 12 patients and normal skin samples of 12 control subjects. Results In normal skin,the positive signals of p-JNK were mostly localized in basal layer cells of the epidermis,with a positive rate of (8.8±1.3)%. In the burned group,the positive signals of p-JNK were mainly localized in the epidermal cells and some inflammatory cells,with a significantly higher positive rate of (31.2±3.3)% than the normal group(P<0.01). Conclusion The changes of p-JNK expression after skin burned might correlate with wound healing.

Key words: forensic pathology, burn injury, JNK mitogen-activated protein kinases, wound healing