法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (4): 241-243.DOI: 10.3969/j.issn.1004-5619.2013.04.001

• 论著 •    下一篇

兔死后角膜厚度与死亡时间的关系

李晓娜1，郑吉龙2，胡忠国3，王保捷4   

  1. （1. 中国医科大学基础医学院，辽宁 沈阳 110001； 2. 中国刑事警察学院法医系，辽宁 沈阳 110854； 3. 吉林省高级人民法院，吉林 长春 130033； 4. 中国医科大学法医学院，辽宁 沈阳 110001）
  • 发布日期:2013-08-25 出版日期:2013-08-28
  • 通讯作者: 王保捷，男，教授，博士研究生导师，主要从事法医物证学教学、科研及鉴定工作；E-mail：bjwang@mail.cmu.edu.cn
  • 作者简介:李晓娜（1980—），女，辽宁沈阳人，博士研究生，主要从事血清学研究；E-mail：xiaona830@mail.cmu.edu.cn

Relationship between Corneal Thickness and Postmortem Interval in Rabbit

LI XIAO-NA1, ZHENG JI-LONG2, HU ZHONG-GUO3, WANG BAO-JIE4   

  1. （1. College of Basic Medical Science, China Medical University, Shenyang 110001, China; 2. Department of Forensic Medicine, China Criminal Police College, Shenyang 110854, China; 3. Superior People’s Count of Jilin Province, Changchun 130033, China; 4. College of Forensic Medicine, China Medical University, Shenyang 110001, China）
  • Online:2013-08-25 Published:2013-08-28

被引次数

7

摘要： 目的 研究兔死后角膜厚度变化规律与死亡时间（postmortem interval，PMI）的关系。 方法 用空气栓塞法建立兔死亡模型。在兔死后0~72 h，每隔6 h进行角膜取材，切片后常规HE染色，用光学显微镜摄取全角膜图像，结合Motic Images Plus 2.0图像分析软件观察角膜上皮层厚度（x1）、角膜基质层厚度（x2）、全角膜厚度（x3） 3项指标的变化值，并作统计学分析，建立与PMI（y）的回归方程。 结果 在兔死后72 h内，角膜基质层厚度和全角膜厚度均在死后12 h开始增加，并在死后54 h达到高峰，两者与PMI正相关，回归方程分别为y=-0.070 2 x22+11.398 x2+1 634（R2=0.712 2，P＜0.05），y=-0.074 9 x32+12.036 x3+ 1 819.4（R2=0.675 0，P＜0.05）。 结论 角膜基质层厚度和全角膜厚度与PMI之间呈较好的线性关系，且角膜基质层厚度优于全角膜厚度，可作为推断死亡时间的指标。

关键词: 法医病理学, 角膜, 死亡时间, 兔

Abstract: Objective To explore the relationship between corneal thickness and postmortem interval （PMI） in rabbit. Methods The rabbit model was established by air embolism. The rabbit cornea was sampled at 6-hour-interval from 0 to 72 h postmortem. After routine HE staining, the whole cornea image was collected by the optical microscope. Three markers were observed including corneal epithelial thickness （x1）, corneal stromal thickness （x2） and whole corneal thickness （x3） using Motic Images Plus 2.0 image analysis software and the data were statistically analyzed to establish the regression function with PMI （y）. Results Within 72 h postmortem, rabbit corneal stromal thickness and whole corneal thickness increased at 12 h postmortem and reached the peak at 54 h postmortem. The two markers showed positive correlation with PMI. The regression functions of the two markers were y= -0.070 2 x22+11.398 x2+1 634 （R2=0.712 2, P＜0.05) and y= -0.074 9 x32+12.036 x3+1 819.4 （R2=0.675 0, P＜0.05), respectively. Conclusion The two markers of corneal stromal thickness and the whole corneal thickness showed the strong linear correlation with PMI. The correlation of the corneal stromal thickness is better than the whole corneal thickness. The two markers can be used to estimate PMI.

Key words: forensic pathology, cornea, postmortem interval, rabbits

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