法医学杂志

法医学杂志 ›› 1994, Vol. 0 ›› Issue (4): 0-0.

• 论文 •    

大鼠死后肝细胞核DNA的组织化学定量研究

涂彬，孔祥麟，阎祖康，顾云菊，陈自强，康健南   

  1. 上海医科大学法医学系，上海铁道医学院中心实验室
  • 发布日期:1994-08-25 出版日期:1994-08-28

A Histochemical Quantitative Study on Nuclear DNA in Rat Liver Cell after Death

TI BIN; KONG XIANGLIN; YAN ZHUKANG; GU YUNJU (FACULTY OF FORENSIC MEDICINE,SHANGHAI MEDICAL UNIVERSITY; SHANGHAI 200032), CHEN ZHIQIANG; KANG JIANNAN (CENTRAL LABORATORY,SHANGHAI RAILWAY MEDICAL COLLEGE;SHANGHAI 200070)   

  • Online:1994-08-25 Published:1994-08-28

被引次数

33

摘要： 推断死亡时间是法医学尸体检验须解决的重要问题之一。本实验用显微分光光度计定量测定了不同环境温度下（１０℃、２０℃、３０℃）大鼠死后不同时间肝细胞核ＤＮＡ平均含量，发现１０℃、２０℃、３０℃下大鼠死后肝细胞核ＤＮＡ含量与死亡时间呈负线性相关，其相关系数分别为—０．９９０５、—０．９８６３、—０．９７０２，且随环境温度升高ＤＮＡ含量的下降速度明显加快。作者认为ＤＮＡ组织化学定量技术可望成为推断死亡时间的新方法。

关键词: 显微分光光度计, DNA, 肝细胞核, 死亡时间

Abstract: Postmortem interval (PMI) estimation is one of the most important problems in forensic autopsy. A microspoetrophotometeric study was conducted on the mean nuclear DNA content in rat liver cell after different time postmortem under different environmental temperature (10℃, 21℃, 30℃), We found rat nuclear DNA content was linear related with PMI under 10℃, 20℃ and 30℃ with correlations of-0. 9905,-0. 9863 and -0. 9702 respectively,and the decrease of nuclear DNA content accelerated under higher environmental temperature. We believe the histochemical quantitatve technique should be a new way to correct estimation of PMI.

Key words: microspectrophotometer, DNA, hepatic cell nuclear, postmortem interval