法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    

广州地区汉族人群Y染色体多个STR位点的研究

唐双柏,郭景元,刘超,梁赏猷,王穗保   

  1. 中山医科大学法医学系,广州市公安局刑事科学技术研究所
  • 发布日期:1999-04-25 出版日期:1999-04-28

Polymorphism and hapletype study of Y-chromosomal multi-STRs loci in Han population in Guangzhou

TANG SHUANGBO, GUO JINGYUAN, LIU CHAO ET AL (DEPARTMENT OF FORENSIC SCIENCES, SUN YAT-SEN UNIVERSITY, GUANGZHOU 510089, P.R.CHINA)   

  • Online:1999-04-25 Published:1999-04-28

被引次数

29

摘要： 为建立一套高度多态且实用性强的Ｙ－ＳＴＲｓ标记系统，本文用ＰＣＲ结合银染显色技术研究了１１１例广州汉族男性ＤＹＳ１９、ＤＹＳ３８９Ⅰ／Ⅱ、ＤＹＳ３９０等位基因及单倍型分布状况。结果显示：广州地区汉族男性ＤＹＳ１９位点有Ａ１、Ａ２、Ａ３、Ａ４、Ａ５五种等位基因，出现频率分别为１８．９％、３６．０％、３６．０％、７．２％、１．８％；ＤＹＳ３８９Ⅰ位点有Ｂ１、Ｂ２、Ｂ３、Ｂ４四种等位基因，出现频率分别为６．３％、５２．２％、１７．１％、２４．３％；ＤＹＳ３８９Ⅱ位点有Ｃ１、Ｃ２、Ｃ３、Ｃ４、Ｃ５五种等位基因，出现频率分别为１１．７％、３８．７％、２２．５％、１７．１％、９．９％，ＤＹＳ３９０位点有Ｄ１、Ｄ２、Ｄ３、Ｄ４、Ｄ５五种等位基因，出现频率分别为２．７％、９．９％、４６．８％、２７．９％、１２．６％；χ２检验表明上述各等位基因分布存在明显的地域差异。此外，还观察到７２种由上述座位共同构成的单倍型，单倍型多样性达０．９８０，表明该系统具有较强的个体识别能力

关键词: Y染色体, 短串联重复序列, 单倍型, 遗传多态性

Abstract: Using polymerase chain reaction and silver stain, polymorphism and hapletype study of Y-chromosomal multi-STRs loci: DYS19, DYS389I/II, DYS390 were studied. 111 samples of male were collected from Guangzhou area. 5 alleles were determined in DYS19 locus, 4 alleles in DYS389I locus, 5 alleles in DYS389II locus and 5 alleles in DYS390 locus. Compared with other racial populations, differences of distribution of allele frequencies existed significantly. 72 hapletype were present in this study.

Key words: Y-chromosome, STR, hapletype, genetic