法医学杂志

法医学杂志 ›› 2001, Vol. 17 ›› Issue (2): 74-76.

• 论文 • 上一篇    下一篇

心肌炎猝死者心、脾中柯萨奇B_3病毒基因的检测

陈玉川,成建定,马会慧,胡丙杰,姚春斓   

  1. 中山医科大学法医病理学教研室!广东广州510080,中山医科大学法医病理学教研室!广东广州510080,中山医科大学附属第三医院传染病科!广东广州510630,中山医科大学法医病理学教研室!广东广州510080,中山医科大学附属第三医院传染病科!广东广州510630
  • 发布日期:2001-04-25 出版日期:2001-04-28

Detection of Coxsackie virus B_3 gene in myocardium and spleen samples in myocarditis with sudden death

CHEN YU－ CHUAN1,CHENG JIAN－ DING1,MA HUI－ HUI2,ET AL.(1.DEPARTMENT OF FORENSIC PATHOLOGY,SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES,GUANGZHOU 510080;2.DEPARTMENT OF INFECTIOUS DISEASES,THIRD AFFILIATED HOSPITAL,SUN YAT－ SEN UNIVERSITY OF MEDICAL SC   

  • Online:2001-04-25 Published:2001-04-28

被引次数

2

摘要： 目的 检测病毒性心肌炎（ viral myocarditis,VMC）猝死者心、脾组织柯萨奇 B_3病毒 (CVB_3)基因，探索 VMC的病原学诊断方法。方法 运用原位逆转录 PCR技术检测实验组（ VMC猝死， 8例）及对照组（非心性死亡， 4例）心、脾组织中的 CVB_3基因。结果 实验组中， 3例（第 1， 4， 7例）心肌 CVB_3基因阳性， 4例（第 2， 4， 6， 7例）脾组织 CVB_3基因阳性；对照组心、脾 CVB_3基因均为阴性。结论 心、脾组织中 CVB_3基因联合阳性可能是 VMC的重要特征；联合检测心、脾中 CVB_3基因可提高 VMC的病原学诊断率。

关键词: 心肌炎, 柯萨奇, B_3病毒, 原位逆转录, PCR

Abstract: Objective To detect the Coxsackie virus B_3(CVB_3) gene in myocardium and spleen tissues in viral myocarditis(VMC) with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene.Methods By in situ RT－ PCR,the detection of CVB_3 gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non－ cardiac death group.Results In VMC group,CVB_3 gene－ positive signals were seen in myocardium sections(3 out of total 8 cases， No.1,4,7 cases)and spleen sections(4 out of total 8 cases,No.2,4,6,7 cases).In non－ cardiac death group,no positive signals were detected in both myocardium and spleen tissues.Conclusion Positive detection of CVB_3 gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC. [

Key words: Coxsackievirus B_3, myocarditis, in situ RT－ PCR