法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (2): 65-67+1.

• 论文 •    下一篇

大鼠急性心肌缺血再灌流损伤c-fos mRNA原位杂交实验研究

马孟云,徐小虎   

  1. 深圳市公安局刑科所,汕头大学医学院 广东 深圳 518008 ,广东 汕头 515031
  • 发布日期:2003-04-25 出版日期:2003-04-28

Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats

MA MENG-YUN,XU XIAOHU (SHENZHEN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE,SHENZHEN 518008,CHINA; COLLEGE OF MEDICAL SCIENCE,SHANTOU UNIVERSITY,SHANTOU 515031,CHINA)   

  • Online:2003-04-25 Published:2003-04-28

被引次数

7

摘要： 目的 探讨急性心肌缺血和/或再灌流早期不同时间不同区域心肌细胞内原癌基因c-fos mRNA表达变化,为心肌早期缺血死后诊断提供依据。方法 复制心肌早期缺血模型,大鼠分为正常、缺血及缺血再灌流组。采用原位杂交方法结合图像分析与统计学处理方法研究心肌细胞内c-fos mRNA表达情况。结果 缺血10min再灌流30min,部分大鼠再灌流区心肌细胞胞浆内c-fos mRNA弱阳性反应,散在分布。缺血60min再灌流30min时达高峰,120min开始减弱。正常和单纯缺血组中缺血少于60min组未见有阳性表达。HE染色无明显病理改变。结论 原位杂交法检测c-fos mRNA方法灵敏特异,有望成为一项急性心肌缺血/再灌流早期损伤的诊断指标。

关键词: 心肌缺血, 再灌流, 原位杂交, c-fos mRNA

Abstract: Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R.

Key words: myocardial ischemia/reperfusion(I/R), c-fos mRNA, in situ hybridization