法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (3): 164-168.DOI: 10.3969/j.issn.1004-5619.2011.03.002

• 论著 • 上一篇    下一篇

中介素对大鼠急性心肌缺血损伤的作用及机制

杜秋香1，岳  维2，王英元1   

  1. （1. 山西医科大学 法医学院法医病理教研室，山西 太原 030001； 2. 山西医科大学 第二医院麻醉研究室，山西 太原 030001）
  • 发布日期:2011-06-25 出版日期:2011-06-28
  • 通讯作者: 通信作者：王英元，男，教授，博士研究生导师，主要从事法医病理学（损伤及死亡时间）研究
  • 作者简介:杜秋香（1982—），女，山西偏关人，博士研究生，主要从事法医病理学研究；E-mail：dqx_2008@126.com
  • 基金资助:

    山西省研究生立项优秀创新项目（20093065）

Effect and Mechanism of Intermedin in Acute Rat Cardiac Ischemic Injury

DU QIU-XIANG1, YUE WEI2, WANG YING-YUAN1   

  1. （1. Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Anesthesiology, the Ｓecond Hospital of Shanxi Medical University, Taiyuan 030001, China）
  • Online:2011-06-25 Published:2011-06-28

被引次数

15

摘要： 目的 探讨中介素（intermedin，IMD）对大鼠急性心肌缺血损伤的作用及机制，为研究心源性猝死机制提供思路。 方法 72只健康雄性大鼠随机分为对照组、缺血组、IMD干预组。酶化学法检测心血中乳酸脱氢酶（lactate dehydrogenase，LDH）、丙二醛（malondialdehyde，MDA）、超氧化物歧化酶（superoxide dismutase，SOD）活性；实时荧光定量PCR法检测心肌降钙素受体样受体（calcitonin receptor-like receptor，CRLR）、受体活性修饰蛋白（receptor activity-modifying proteins，RAMPs）的mRNA表达水平；酶联免疫吸附法测定心肌环磷酸腺苷（cyclic adenosine monophosphate，cAMP）的含量，免疫组化法检测Bcl-2和Bax蛋白表达。 结果 与对照组比较，缺血组大鼠心血中LDH、MDA活性升高，SOD活性下降，心室肌中cAMP含量增加，Bcl-2与Bax蛋白表达量比值降低；静脉给予IMD干预后，大鼠缺血后LDH、MDA活性升高程度降低，SOD活性降低程度减少，心室肌CRLR、RAMPs的mRNA水平均明显上调，心室肌中cAMP含量增加更显著，而Bcl-2与Bax蛋白表达量比值降低程度减少（P<0.05）。 结论 IMD通过减少缺血所致的氧化应激，抑制心肌细胞凋亡，对大鼠急性心肌缺血损伤有明显的保护作用。

关键词: 法医病理学, 心肌缺血, 中介素, 大鼠

Abstract: Objective To investigate the effect and potential mechanism of intermedin（IMD） in acute cardiac ischemic injury and to provide a new approach for exploring mechanism of sudden cardiac death. Methods Seventy-two healthy male rats were randomly divided into 3 groups： control, ischemic and the IMD-treated group. The activity of lactate dehydrogenase（LDH）, malondialdehyde（MDA） and superoxide dismutase（SOD）  in heart blood were tested by enzyme chemistry method. The mRNA changes of calcitonin receptor-like receptor（CRLR） and receptor activity-modifying proteins（RAMPs） in cardiac were measured by real-time PCR analysis. Myocardial cyclic adenosine monophosphate（cAMP） content was determined by enzyme linked immunosorbent assay（ELISA）. Apoptosis related factors Bcl-2 and Bax were detected by immunohistochemistry. Results Comparing with the control group, LDH and MDA activity of ischemic group in heart blood increased and SOD activity decreased. The concentration of cAMP increased in ventricular muscle, Bcl-2 and Bax proteins expression ratio level decreased. The intravenation of IMD decreased the level of increased activity of LDH and MDA, and lessened the level of decreased activity of SOD. The mRNA expression of CRLR and RAMPs obviously increased in ventricular muscle. Conclusion The protective effect of IMD against myocardial ischemic injury could be caused by decreasing the oxidative stress of ischemia and inhibiting the myocardial apoptosis.

Key words: forensic pathology, myocardial ischemia, intermedin, rats

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