法医学杂志 ›› 2003, Vol. 0 ›› Issue (2): 76-77.

• 论文 • 上一篇    下一篇

家兔角膜上皮和内皮细胞DNA含量与PMI关系的图像分析

刘承泉,熊平   

  1. 南华大学法医病理教研室,南华大学法医病理教研室 湖南 衡阳 421001 ,湖南 衡阳 421001
  • 发布日期:2003-04-25 出版日期:2003-04-28

The relationship between the postmortem interval and the changes of DNA content in rabbit's cornea epithelial and endothelium by the computerized image analysis

LIU CHENG-QUAN,XIONG PING (DEPARTMENT OF FORENSIC MEDICINE,NANHUA UNIVERSITY,HENGYANG 421001,CHINA)   

  • Online:2003-04-25 Published:2003-04-28

摘要: 目的 探讨家兔角膜上皮和内皮细胞DNA含量与PMI的关系。方法 应用计算机图像分析技术,测定105只家兔死后72h内不同时间点家兔角膜上皮和内皮细胞核DNA含量(IOD)的变化值并做统计学分析。结果 家兔死后72h内其角膜上皮细胞和内皮细胞核DNA降解速率与PMI具有显著的相关性,并由此归纳出体现DNA降解趋势的二项式回归方程。结论 应用计算机图像分析技术,测量机体死后角膜内外皮细胞DNA含量变化,将会成为推断PMI精确、客观的新方法。

关键词: 死后经过时间, PMI图像分析, 家兔, 角膜, DNA含量

Abstract: Objective To study the relationship between the postmortem interval (PMI)and the changes of DNA content in rabbit's cornea epithelial and endothelium. Methods Using the computerized image analysis to measure the DNA content in the cornea epithelial and endothelium of 105 rabbit's at every six hours during 72 hours after death . Results The degradation rate of DNA in nuclear has an apparent relationship in 72 hours after death of the rabbits. Conclusion The determination of the quantity of DNA in cornea nuclear is a new method to estimate the PMI accurately.

Key words: postmortem interval, image analysis, rabbit, cornea, DNA content