法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 72-73.

• 论文 • 上一篇    下一篇

陈旧骨骼DNA提取

张杰;薛庆节;唐光峰;彭磊;   

  1. 泰安市公安局刑警支队,济宁医学院微生物教研室,泰安市公安局刑警支队,泰安市公安局刑警支队 山东泰安271000,山东济宁272400,山东泰安271000,山东泰安271000
  • 发布日期:2006-02-25 出版日期:2006-02-28

DNA Extraction from Old Bones

ZHANG JIE1, XUE QING-JIE2, TANG GUANG-FENG1, PENG-LEI1 (1.CRIMINAL POLICE DEPARTMENT OF TAIAN CITY PUBLIC SECURITY BUREAU, TAIAN 271000,CHINA; 2.JINING MEDICINAL COLLEGE,JINING 272400,CHINA)   

  • Online:2006-02-25 Published:2006-02-28

摘要： 目的寻找从陈旧骨骼中提取DNA的有效方法。方法运用传统的有机法结合Microcon100纯化柱提取骨骼DNA。结果用常规荧光标记复合STR基因分型法可对提取到的陈旧骨骼DNA进行成功分析。结论有机法结合Micrcon100纯化柱提取陈旧骨骼DNA法可有效应用于实际检案。

关键词: 有机法, Microcon100, DNA提取, 陈旧骨骼

Abstract: Objective To explore an efficient method for extracting DNA from old bones. Methods Using an organic combined with and Microcon 100 to extract and purify DNA. Results The extracted DNA was successfully genotyped by using florescence labeling STR multiplex amplification. Conclusion This method will be useful for forensic scientists in identification of DNA from old bones.

Key words: organic method, microcon100, DNA extraction, old bones