法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 122-124.

• 论文 • 上一篇    下一篇

荧光复合扩增检测3个Y-STR基因座单倍型

林晓燕;王磊;原宝龙;冯常俊;黄代新;   

  1. 华中科技大学同济医学院法医学系,郑州市公安局刑侦支队,郑州市公安局刑侦支队,郑州市公安局刑侦支队,华中科技大学同济医学院法医学系 湖北武汉430030,河南郑州450003,河南郑州450003,河南郑州450003,湖北武汉430030
  • 发布日期:2006-04-25 出版日期:2006-04-28

Detecting the Haplotype of Three Y-STR Loci by Fluorescent Multiplex Amplification

LIN XIAO-YAN1, WANG LEI2, YUAN BAO-LONG2, FENG CHANG-JUN2,HUANG DAI-XIN1 (1.FACULTY OF FORENSIC MEDICINE, TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY, WUHAN 430030, CHINA; 2.ZHENGZHOU PUBLIC SECURITY BUREAU,ZHENGZHOU 450003,CHINA   

  • Online:2006-04-25 Published:2006-04-28

被引次数

8

摘要： 目的建立检测3个Y-STR基因座Y-GATA-A7.1、DYS456和DYS443的荧光复合扩增体系,并获取中国汉族人群单倍型频率分布。方法用荧光标记引物对郑州地区203名汉族男性无关个体进行3个基因座复合扩增,ABI3100型遗传分析仪检测、分型。结果Y-GATA-A7.1、DYS456和DYS443基因座分别检出5、6和6个等位基因,其基因多样性(GD值)分别为0.6692、0.5839和0.7053。三个基因座构成的单倍型共有44种,单倍型多样性(HD值)为0.9523。结论建立的3个Y-STR基因座荧光标记复合扩增系统具有很高的识别能力,可应用于法医学实践。

关键词: Y-STRs, 复合扩增, 单倍型, Y-GATA-A7.1, DYS456, DYS443

Abstract: Objective To establish a fluorescent multiplex PCR system for typing Y-STR loci Y-GATA-A7.1, DYS456 and DYS443, and investigate their haplotype frequencies in Chinese Han population. Methods 203 unrelated males of Han population living in Zhengzhou were typed by fluorescent multiplex amplification system and ABI 3100 genetic analyzer. Results In Zhengzhou Han population, 5,6 and 6 different alleles were observed for Y-GATA-A7.1, DYS456 and DYS443 loci, and their gene diversity (GD) were 0.669 2, 0.583 9 and 0.705 3 respectively. A total of 44 different haplotypes formed by these three loci was identified and the haplotype diversity (HD) reached 0.952 3. Conclusion The fluorescent multiplex system for these three Y-STR loci will be very powerful for forensic individual identification and paternity testing in Chinese Han population.

Key words: Y-STRs, multiplex amplification, haplotype, Y-GATA-A7.1, DYS456, DYS443