法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (1): 18-19+2.

• 论文 • 上一篇    下一篇

实验性大鼠脑震荡后Bcl-2的表达

朱旭阳;汪枫;方维华;吴茂旺;   

  1. 皖南医学院法医系,皖南医学院法医系,皖南医学院法医系,皖南医学院法医系 安徽芜湖241001,安徽芜湖241001,安徽芜湖241001,安徽芜湖241001
  • 发布日期:2007-02-25 出版日期:2007-02-28

Bcl-2 Expression Following the Brain Concussion in Rats

ZHU XU-YANG,WANG FENG,FANG WEI-HUA,WU MAO-WANG (DEPARTMENT OF FORENSIC MEDICINE,WANNAN MEDICAL COLLEGE,WUHU 241001,CHINA)   

  • Online:2007-02-25 Published:2007-02-28

被引次数

12

摘要： 目的观察实验性大鼠脑震荡后Bcl-2表达的变化规律。方法应用免疫组织化学方法检测大鼠脑震荡后不同时间大脑皮层、脑干和小脑等脑区内Bcl-2表达的变化规律进行研究。结果对照组大鼠未见Bcl-2蛋白的表达。脑震荡组损伤后1h组可在神经细胞内观察到少量Bcl-2蛋白的表达,4d达到高峰,8d和16d,Bcl-2蛋白表达随后逐渐下降。结论Bcl-2的检测可能成为脑震荡的诊断和脑损伤时间推断的一个敏感指标。

关键词: 脑震荡, Bcl-2, 免疫组织化学

Abstract: Objective To evaluate the expression of Bcl-2 protein after brain concussion.Methods Expression levels of Bcl-2 protein in cortex,pontine and cerebellum of rats were investigated using immunohistochemistry.Results There was no expression of Bcl-2 protein in control group seen.The expression of Bcl-2 protein in brain concussion groups was detected at 1hour,and the expression level reached its peak 4 days after the concussion and then declined gradually.Conclusion Our findings suggest that the detection of Bcl-2 protein could be an indicator for diagnosis of brain concussion and for estimation of the post injury time interval.

Key words: brain concussion, Bcl-2, immunohistochemistry