法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (2): 86-89+1.

• 论文 • 上一篇    下一篇

毒鼠强中毒大鼠脑GABA及GABA_AR-α1的表达

陶涛;陈伟杰;潘洪富;李斌;廖志钢;   

  1. 四川大学华西基础医学与法医学院,四川大学法学院 四川成都610041,四川大学华西基础医学与法医学院,余姚市公安局,浙江余姚315400,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,,四川成都610041,四川成都610041,四川成都610041,四川成都610041,四川成都610041
  • 发布日期:2007-04-25 出版日期:2007-04-28

Expressions of GABA and GABA_AR-α1 in the Brain of Rats Poisoned by Tetramine

TAO TAO1,2,CHEN WEI-JIE1,3,PAN HONG-FU1,LI BIN1,LIAO ZHI-GANG1(1.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE,SICHUAN UNIVERSITY,CHENGDU 610041,CHINA;2.LAW SCHOOL,SICHUAN UNIVERSITY,CHENGDU 610041,CHINA;3.BUREAU OF PUBLIC SECURITY,YUYAO 315400,C   

  • Online:2007-04-25 Published:2007-04-28

摘要： 目的探讨毒鼠强中毒大鼠脑GABA及GABAAR-α1表达的变化及可能机制。方法健康Sprague-Dawley(SD)大鼠60只,随机分为正常对照组、空白对照组、高剂量中毒组和低剂量中毒组,每组5只。高剂量中毒组每只以1.0mg/kg的剂量用毒鼠强悬浊液灌胃,低剂量中毒组每只以0.1mg/kg的剂量用毒鼠强溶液灌胃制作中毒模型。应用免疫组化技术和图像分析仪对GABA及GABAAR-α1的表达情况和变化规律进行研究。结果(1)正常大鼠脑组织内GABA及GABAAR-α1表达较为广泛,维持在中等水平;(2)高剂量中毒组脑内GABA及GABAAR-α1表达均下降;(3)低剂量中毒组脑内GABA表达先下降,于中毒后6h降至最低,后表达开始增加,3d时接近对照组水平,5～7d达高峰,10d时仍高于对照组;(4)低剂量中毒组脑内GABAAR-α1表达先下降,于中毒后6～12h降至最低,后表达开始增加,7～10d时接近对照组水平。结论(1)高剂量毒鼠强中毒情况下,GABA及GABAAR-α1表达均下降;(2)大鼠脑GABA及GABAAR-α1表达改变与毒鼠强中毒的发生机制密切相关。

关键词: GABA, GABAAR, 毒鼠强, 免疫组织化学

Abstract: Objective Expressions of GABA and GABAAR-α1 in the brain of rats poisoned by Tetramine were analyzed to explore the intoxication mechanism.Methods Sixty rats were randomly divided into control,sham poisoned,high-dose poisoned(1.0 mg/kg tetramine) and low-dose poisoned(0.1 mg/kg) groups.The expressions of GABA and GABAAR-α1 in the brain of the poisoned rats were detected and analyzed by immunohistochemistry and imaging analyzer.Results The expressions of both GABA and GABAAR-α1 were diffusely seen in the brains of the control and shame poisoned rat groups with a moderate expression level,whereas the expressions of both GABA and GABAAR-α1 were decreased in the brains of the high-dose poisoned group.In the low-dose poisoned rat group,the expression of GABA initially decreased and reached its lowest level 6 hours after poisoning,and then started to show an increase and reached the level of control groups at day 3.The expressions level reached its peak at days 5-7 after poisoning and remained above the level of control groups till 10 days after poisoning.Similarly,the expression of GABAAR-α1 in the brains of the low-dose poisoned group initially decreased and reached its lowest level 6-12 hrs after poisoning,and then started to increase and reached the level of control groups at days 7-10 after poisoning,respectively.Conclusion The expression of both GABA and GABAAR-α1 decreased in the brains of the high-dose poisoned rat group and these changes of GABA and GABAAR-α1 expressions may be associated with underlying mechanism of tetramine poisoning.

Key words: GABA, GABAAR-α1, tetramine, immunohistochemistry