法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (3): 188-190.

• 论文 • 上一篇    下一篇

皮肤成纤维细胞纤维连接蛋白及其EDA、EDB表达与损伤时间的关系

余松;薛爱民;贺盟;赵子琴;沈忆文;顾云菊;   

  1. 湖州市公安局,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系 浙江湖州313000,上海200032,上海200032,上海200032,上海200032,上海200032
  • 发布日期:2007-06-25 出版日期:2007-06-28

The Relationship Between Fibronectin EDA/EDB mRNA in Skin Fibroblast and Injury Time

YU SONG1, XUE AI-MIN2, HE MENG2, ZHAO ZI-QIN2, SHEN YI-WEN2, GU YUN-JU2(1.HUZHOU PUBLIC SECURITY BUREAU, HUZHOU 313000, CHINA; 2.DEPARTMENT OF FORENSIC MEDICINE, SHANGHAI MEDICAL COLLEGE, FUDAN UNIVERSITY, SHANGHAI 200032,CHINA)   

  • Online:2007-06-25 Published:2007-06-28

被引次数

20

摘要： 目的研究培养的皮肤成纤维细胞纤维连接蛋白(fibronectin,FN)及其可变剪接片段EDA、EDB在损伤后的mRNA水平,为推断早期损伤时间提供参考指标。方法培养人体皮肤成纤维细胞,换无血清培养液培养24h后,采用划痕法制作细胞损伤模型,损伤后0、0.5、1、2、6、24h收集细胞,抽提RNA,实时RT-PCR定量检测总FN及其EDA、EDB的mRNA水平的变化。结果皮肤成纤维细胞中总FN及其EDA、EDB的mRNA水平随损伤时间的延长而升高,EDA mRNA在损伤后1h达到高峰,EDB mRNA在损伤后6h达到高峰,FN mRNA在损伤后2h达到高峰;在损伤1h后总FN及其EDA、EDB的mRNA表达变化随时间的延长而呈现一种不断上升的趋势。结论纤维连接蛋白EDA、EDB可作为早期损伤时间推断的较理想的参考指标。

关键词: 成纤维细胞, 纤维连接蛋白, EDA, EDB, 早期损伤时间推断, 实时RT-PCR

Abstract: Objective In order to effectively estimate early injury time, The transcription of fibronectin and alternative splicing segment of fibronectin-EDA/EDB were studied. Methods The human skin fibroblast cells were cultured and incubated in serum-free medium for 24 h. The cells were injured with scratch technique. The cells were harvested at 0, 0.5, 1, 2, 6 and 24 h after injuries. Total RNA was extracted and real-time RT-PCR was performed to quantify mRNAs for fibronectin, EDA and EDB. Results The mRNA level of fibronectin, EDA and EDB were increased after injury and showed a gradually increased tendency. The EDA mRNA peaked in 1h after injury, EDB mRNA 6 h, fibronectin mRNA 2 h. Conclusion FN-EDA/EDB may be used as a sensitive marker for the estimation of early injury time.

Key words: fibroblast, fibronectin, EDA, EDB, early injury time estimation, real-time RT-PCR