法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (6): 409-412.DOI: 10.3969/j.issn.1004-5619.2014.06.001

• 论著 •    下一篇

EIIIA+纤维连接蛋白在大鼠皮肤切创的表达

贺  盟1，赵  海2，沈忆文1，薛爱民1，陈  龙1，顾云菊1，赵子琴1   

  1. （1. 复旦大学基础医学院法医学系，上海 200032； 2. 上海市公安局水上公安分局，上海 200002）
  • 发布日期:2014-12-25 出版日期:2014-12-28
  • 作者简介:贺盟（1975—），男，山东临清人，硕士，主要从事法医病理学和法医临床学研究；E-mail：042101055@fudan.edu.cn

Expression of EIIIA+ Fibronectin in Incised Wound of Rat’s Skin

HE MENG1, ZHAO HAI2, SHEN YI-WEN1, XUE AI-MIN1, CHEN LONG1, GU YUN-JU1, ZHAO ZI-QIN1   

  1. （1. Department of Forensic Medicine, School of Basic Medical Science, Fudan University, Shanghai 200032, China; 2. Harbor Public Security Subbureau, Shanghai Public Security Bureau, Shanghai 200002, China）
  • Online:2014-12-25 Published:2014-12-28

被引次数

1

摘要：  目的 探讨大鼠皮肤切创纤维连接蛋白EIIIA片段的表达与损伤时间的关系。 方法 取48只成年SD大鼠并切割大鼠背部皮肤致伤。于伤后即刻、0.5 h、1 h、2 h、3 h、4 h、6 h、8 h共8个时间段分别处死大鼠，取其创伤周围皮肤，利用免疫组织化学方法和Western印迹法检测EIIIA+纤维连接蛋白，观察其表达水平与损伤时间的关系。 结果 伤后即刻未出现EIIIA+纤维连接蛋白的表达，伤后0.5 h，皮肤基底细胞开始出现阳性表达，随损伤时间的延长，阳性着色持续增强。Western印迹法检测发现，所测得的相对光密度值随损伤时间的延长而逐渐增高。 结论 EIIIA片段表达变化可以作为法医学早期损伤时间的推断指标。

关键词: 法医病理学, 创伤和损伤, 皮肤, 纤维连接蛋白, 大鼠

Abstract: Objective To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat’s skin and injury time. Methods The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA+ fibronectin was detected by immunohistochemistry and Western blotting and the relationship between its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis. Conclusion The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.

Key words:  forensic pathology, wounds and injuries, skin, fibronectin, rats

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