法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (4): 246-249.DOI: 10.3969/j.issn.1004-5619.2011.04.002

• 论著 • 上一篇    下一篇

小鼠皮肤切创愈合过程中纤维细胞募集和分化的时间依赖性

范琰琰1，官大威1，王  涛1，刘伟伟1，郑吉龙1，2，赵振宾1，于天水1，马文翔1   

  1. （1. 中国医科大学 法医学院，辽宁 沈阳 110001； 2. 中国刑警学院 法医学系，辽宁 沈阳 110854）
  • 发布日期:2011-08-25 出版日期:2011-08-28
  • 通讯作者: 通信作者：官大威，男，教授，博士研究生导师，主要从事皮肤损伤愈合机制及钝力性心脏外伤研究；E-mail：dwguan@mail.cmu.edu.cn
  • 作者简介:范琰琰（1982—），男，安徽六安人，博士研究生，主要从事皮肤损伤愈合机制的研究；E-mail：yyfan998@yahoo.com

Time-dependent Recruitment and Differentiation of Fibrocytes in Mouse Skin Wound Healing

FAN YAN-YAN1, GUAN DA-WEI1, WANG TAO1, LIU WEI-WEI1, ZHENG JI-LONG1,2, ZHAO ZHEN-BIN1, YU TIAN-SHUI1, MA WEN-XIANG1   

  1. （1. School of Forensic Medicine, China Medical University, Shenyang 110001, China; 2. Department of Forensic Medicine, China Criminal Police University, Shenyang 110854, China）
  • Online:2011-08-25 Published:2011-08-28

被引次数

6

摘要： 目的 观察小鼠皮肤切创愈合过程中，纤维细胞募集和分化随时间变化的规律性。 方法 应用免疫荧光技术共定位纤维细胞［共表达CD45、Ⅰ型前胶原（procollagen Ⅰ）］和肌成纤维细胞［共表达CD45、平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）］，计数纤维细胞及其分化成的肌成纤维细胞。 结果 纤维细胞于切创后3 d募集于创口局部，其分化成的肌成纤维细胞于切创后5 d开始出现于肉芽区。纤维细胞及其分化成的肌成纤维细胞于伤后7 d达到峰值。 结论 在小鼠皮肤切创愈合过程中，肉芽组织中的肌成纤维细胞至少部分来源于纤维细胞的分化，纤维细胞时间依赖性地募集和分化可以为损伤时间的推断提供新的信息。

关键词: 法医病理学, 创伤和损伤, 皮肤, 纤维细胞, 肌成纤维细胞, 损伤时间, 小鼠

Abstract: Objective To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing. Methods Fibrocytes （expressing CD45 and procollagen Ⅰ） and myofibroblasts （expressing CD45 and α-SMA） were co-localized by immunofluorescent staining. The number of fibrocytes and myofibroblasts was counted at different post-wounding interval. Results At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding. Conclusion During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibrocytes may provide new information for wound age determination.

Key words: forensic pathology, wounds and injuries, skin, fibrocyte, myofibroblast, wound age, mice

中图分类号: