法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (3): 193-195+.

• 论文 • 上一篇    下一篇

大鼠皮肤降钙素基因相关肽表达与损伤时间的相关性

周建清;王永忠;蒋红艳;孔杰;   

  1. 镇江市公安局刑事科学技术研究所,江苏大学医学院法医学教研室,镇江市公安局刑事科学技术研究所,镇江市丹徒区公安局刑事科学技术室 江苏镇江212001,江苏镇江212001,江苏镇江212001,江苏镇江212003
  • 发布日期:2007-06-25 出版日期:2007-06-28

Expression of CGRP in Rat's Skin and Post-traumatic Interval

ZHOU JIAN-QING1,WANG YONG-ZHONG2, JIANG HONG-YAN1, KONG JIE3(1.THE CRIMINAL TECHNIQUE INSTITUTE OF ZHENJIANG PUBLIC SECURITY BUREAU, ZHENJIANG 212001, CHINA; 2.DEPARTMENT OF FORENSIC MEDICINE, MEDICAL COLLEGE OF JIANGSU UNIVERSITY, ZHENJIANG 212001,CHINA;   

  • Online:2007-06-25 Published:2007-06-28

被引次数

1

摘要： 目的探讨创伤皮肤组织降钙素基因相关肽(CGRP)的时间相关性表达,为损伤时间推断提供实验依据。方法应用免疫组化技术、图像分析技术和原位分子杂交技术检测不同损伤时间大鼠皮肤组织生前及死后CGRP表达。结果生前组大鼠损伤后皮肤组织内CGRP在神经纤维中表达明显高于对照组(P<0.05)。死后组皮肤组织中CGRP表达与正常对照组比较无明显变化(P>0.05)。生前组中CGRP表达在损伤后1h明显增多,4h达到高峰,以后恢复到正常。结论损伤组织神经纤维中CGRP表达具有规律性且有较好的时间相关性,可成为法医学损伤时间推断的指标。

关键词: 损伤时间, 降钙素基因相关肽(CGRP), 免疫组织化学, 原位杂交

Abstract: Objective To study the expression of calcitonin gene-related peptide (CGRP) in rat's skin after injury. Methods Immunohistochemistry technique, auto-image analysis, and in situ hybridization were used to study the expression of CGRP protein and mRNA at different post-traumatic time intervals in both antemortem and postmortem groups. Results The expression of CGRP in neurofibrils was higher than that in the control (P<0.05). However, in postmortem injury group, there were no significant changes in anti-CGRP immounreactivities in neurofibrils compared to the control group (P>0.05). Further, the expression of CGRP could be detected increasing in 1h, and reached maximum in 4 h, then returned to normal. Conclusion The expression of CGRP in rat's skin is time dependent after skin injury. It could used as a bio-marker to determine the time of injury.

Key words: time of injury, calcitonin gene-related peptide (CGRP), immunohistochemistry, in situ hybridization