法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (1): 42-43.

• 论文 • 上一篇    下一篇

3种方法联合运用提取人体脱落上皮细胞DNA

郭燕霞;陈松;刘开会;   

  1. 公安部物证鉴定中心;
  • 发布日期:2009-02-25 出版日期:2009-02-28

The Application of Three Methods Combined for Extracting DNA from Human Epithelium

GUO YAN-XIA, CHEN SONG, LIU KAI-HUI (FORENSIC SCIENCE INSTITUTE OF PUBLIC SECURITY MINISTRY, BEIJING 100038, CHINA)   

  • Online:2009-02-25 Published:2009-02-28

被引次数

13

摘要： 目的寻求提高法医物证检验中附有人体脱落细胞载体检材的DNA检出率的方法。方法根据案件检材的具体条件,选择3种提取方法即Chelex-100法、Chelex-100联合有机法和Chelex-100联合磁珠法。结果采用了分步提取DNA法,可充分、有效地提取微量DNA,提高了人体脱落上皮细胞有效DNA的检出率。结论根据第一步提取方法的检测结果,来调整下一步的提取策略,如是否加以纯化浓缩,可增加人体脱落上皮细胞有效DNA的检出率。

关键词: 法医遗传学, 提取法, 脱落上皮细胞

Abstract: Objective To improve DNA extraction from human epithelium. Methods Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case. Results With the stepwise extracting protocol, the trace amount of DNA was ana-lyzed most efficiently and the success rate of DNA extracting was better improved. Conclusion The effi- ciency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.

Key words: forensic genetics, extraction, exfoliated epithelial cell