法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (2): 100-103.

• 论文 • 上一篇    下一篇

判别分析法在胃癌组织身源鉴定中的应用

李成涛;赵书民;张素华;李莉;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学生命科学学院;苏州大学医学部法医学系;
  • 发布日期:2010-04-25 出版日期:2010-04-28

Source Identification of Gastric Cancer Tissue with Discriminatory Analysis

LI CHENG-TAO1,2,ZHAO SHU-MIN1,ZHANG SU-HUA1,3,LI LI1 (1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA; 2. SCHOOL OF LIFE SCIENCES,FUDAN UNIVERSITY,SHANGHAI 200433,CHINA; 3.   

  • Online:2010-04-25 Published:2010-04-28

被引次数

9

摘要： 目的评估基于共有基因座数或共有等位基因数的判别分析方法在胃癌组织身源鉴定中的应用价值。方法采用Identifiler试剂盒对22对新鲜的胃癌组织块及相应身源正常组织块进行STR分型,采用计数法获得胃癌组织中各基因座不同变异类型的变异率和胃癌-身源正常组织对中的全不同基因座数(A0)、半相同基因座数(A1)、全相同基因座数(A2)和共有等位基因数(IAn),将上述参数代入已知的Fisher判别函数,以误判率评价Fisher判别函数对胃癌组织身源认定的效果。结果22例胃癌组织中,STR基因型改变(STRgenotypic alteration,STRGA)的发生率为3.03%(95%CI:1.46%～5.50%),至少有一个STR基因座出现STRGA者占31.38%(95%CI:13.86%～54.87%)。采用基于共有基因座数或共有等位基因数的Fisher判别函数,本组胃癌组织均被认定与相应正常组织来自同一个体,误判率为0.00%。结论胃癌组织中STR基因型改变的发生率较高;基于共有基因座数或共有等位基因数的判别函数适用于胃癌组织的身源认定。

关键词: 法医遗传学, 胃肿瘤, 判别分析, 共有基因座, 共有等位基因, 个体识别

Abstract: Objective To evaluate discriminatory analysis on source identification of gastric cancer tissue based on the number of matched STR locus or identical allele. Methods Twenty two pairs of fresh gastric cancer tissue and homologous normal tissue were genotyped with Identifilerkit. Frequencies of STR genotypic alteration(STRGA),the number of matched STR locus without identical allele(A0),with 1 identical allele(A1),or with 2 identical alleles(A2) and the number of total identical alleles(IAn) were calculated with counting method. A1,A2 and IAn were evaluated with Fisher discriminant functions to determine the source of each gastric cancer tissue. Effectiveness of the identification of gastric cancer tissue was evaluated with error rate. Results The total frequency STRGA was 3.03%(95% CI:1.46%-4.88%). There were 31.38%(95% CI:13.86%-54.87%) of gastric cancer samples carried at least one STR locus with STRGA. It was confirmed by the Fisher discriminant functions that each of the 22 gastric cancer tissue samples came from its homologous normal tissue with an error rate of 0.00%. Conclusion Frequency of STRGA in gastric cancer tissue was high. Fisher discriminant functions based on the number of identical alleles or matched STR loci could be a feasible method for source identification of body for gastric cancer tissue samples.

Key words: forensic genetics, stomach neoplasms, discriminant analysis, matched locus, identical allele, personal identification