法医学杂志 ›› 2015, Vol. 31 ›› Issue (4): 257-261.DOI: 10.3969/j.issn.1004-5619.2015.04.001

• 论著 •    下一篇

高温环境下死亡大鼠肾傅里叶显微红外光谱变化

王志军1,2,申山山3,邓恺飞2,秦志强2,黄  平2,王振原1   

  1. (1. 西安交通大学医学部法医学院,陕西 西安 710061; 2. 司法部司法鉴定科学技术研究所 上海市法医重点实验室,上海 200063; 3. 保定市公安局南市区分局,河北 保定 071000)
  • 发布日期:2015-08-25 出版日期:2015-08-28
  • 通讯作者: 黄平,男,博士,副研究员,副主任法医师,主要从事法医病理学研究及鉴定;E-mail:huangpingifs@gmail.com 王振原,男,教授,博士研究生导师,主要从事法医病理学研究及鉴定;E-mail:wzy218@mail.xjtu.edu.cn
  • 作者简介:王志军(1989—),男,河北衡水人,硕士研究生,主要从事法医病理学研究及鉴定;E-mail:wangzhijun@stu.xjtu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目(81273335、81273339)

Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fatal Hyperthermia

WANG ZHI-JUN1,2, SHEN SHAN-SHAN3, DENG KAI-FEI2, QIN ZHI-QIANG2, HUANG PING2, WANG ZHEN-YUAN1   

  1. (1. Department of Forensic Science, Health Science Center, Xi′an Jiaotong University, Xi′an 710061, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 3. Nanshi District of Baoding Public Security Bureau, Baoding 071000, China)
  • Online:2015-08-25 Published:2015-08-28

摘要: 目的 应用傅里叶显微红外光谱(Fourier transform infrared microspectroscopy,FTIR-MSP)技术分析高温死亡大鼠肾的化学基团变化,为高温死亡鉴定提供新的途径与方法。 方法 建立不同死亡原因动物模型并分组,包括高温死亡组、脑干损伤死亡组、失血性休克死亡组、窒息死亡组,大鼠死亡后立即提取其肾组织,使用FTIR-MSP技术采集肾小球区域的红外光谱。 结果 高温死亡组大鼠肾小球组织在3 290、3 070、2 850、1 540及1 396 cm-1处吸收峰强度增高(P<0.05),A1 650/A3 290与A1 650/A1 540的比值降低(P<0.05)。 结论 应用FTIR-MSP技术分析肾组织化学基团变化可作为高温死亡鉴定的辅助诊断方法。

关键词:  , 法医病理学, 谱学, 傅里叶变换红外, 死亡原因, 高温, 肾, 大鼠

Abstract: Objective To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy (FTIR-MSP) and to provide a new method to diagnose fatal hyperthermia. Methods Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP. Results The absorbances of 3 290, 3 070, 2 850, 1 540 and 1 396 cm-1 significantly increased (P<0.05), and the ratios of A1 650/A3 290 and A1 650/A1 540 significantly decreased (P<0.05) in group of hyperthermia. Conclusion FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death.

Key words: forensic pathology, spectroscopy, Fourier transform infrared, cause of death, hyperthermia, kidney, rats

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