法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (2): 105-108.DOI: 10.3969/j.issn.1004-5619.2016.02.007

• 论著 • 上一篇    下一篇

STR基因座与Amelogenin的峰面积比在DNA降解评估中的应用

解雅玲1，李  璐2，邵诚臣1，吴轶慧1，杜铁帅1，周怀谷3，李  辉3，谢建辉1，沈忆文1   

  1. （1. 复旦大学基础医学院法医系，上海 200032； 2. 上海市公安局静安分局，上海 200040； 3. 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083）
  • 发布日期:2016-04-25 出版日期:2016-04-28
  • 通讯作者: 沈忆文，女，博士，副教授，主要从事法医学教学、科研及鉴定；E-mail：shenyiwen@fudan.edu.cn
  • 作者简介:解雅玲（1990—），女，硕士研究生，主要从事法医病理学及法医临床学研究；E-mail：13211010068@fudan.edu.cn
  • 基金资助:

    上海市现场物证重点实验室基金资助项目（XCWZ20）

Application of the Peak Area Ratio of STR Loci to Amelogenin Locus in the Estimation of DNA Degradation

XIE YA-LING1, LI LU2, SHAO CHENG-CHEN1, WU YI-HUI1, DU TIE-SHUAI1, ZHOU HUAI-GU3, LI HUI3, XIE JIAN-HUI1, SHEN YI-WEN1   

  1. （1. Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; 2. Jing’an Branch of Shanghai Public Security Bureau, Shanghai 200040, China; 3. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China）
  • Online:2016-04-25 Published:2016-04-28

摘要： 目的 研究DNA降解过程中STR基因座与性别基因座Amelogenin（AMEL）峰面积比（STR/AMEL）的变化规律，探讨STR/AMEL值在评估DNA降解程度中的应用。 方法 取人体髂腰肌组织抽提DNA，分析DNaseⅠ酶人工降解后STR/AMEL值（Penta E/AMEL、Penta D/AMEL、FGA/AMEL）的变化，并分析室外环境自然降解的髂腰肌组织中三者比值的变化。以降解时间为自变量（x），STR/AMEL值为因变量（y），进行回归曲线分析，建立两种条件下三组曲线方程。 结果 人工降解及自然降解条件下STR/AMEL值与降解时间均呈负相关，一元三次方程能够较好地模拟STR/AMEL值随降解时间的变化规律。人工降解条件下，R2均大于0.99；自然降解条件下，R2均大于0.86。 结论 STR/AMEL值（Penta E/AMEL、Penta D/AMEL、FGA/AMEL）与DNA降解程度呈负相关，有望应用于DNA降解程度的评估。

关键词: 法医遗传学, DNA降解, 坏死, 短串联重复序列, 峰面积

Abstract: Objective To explore the change rules of peak area ratio of STR loci to Amelogenin （AMEL） locus （STR/AMEL）, a sex-determining gene in DNA degradation, and to evaluate the application of STR/AMEL value in the estimation of DNA degradation degree. Methods DNA was extracted from iliopsoas, and the variations of STR/AMEL value （Penta E/AMEL, Penta D/AMEL, FGA/AMEL） were analyzed after the artificial degradation was made by DNaseⅠ, and the changes of these three ratios of the iliopsoas naturally degraded in an outdoor environment were also analyzed. The regression curves were analyzed using the periods of DNA degradation and outside the body as the independent variable （x） and the STR/AMEL value as the dependent variable （y） and three curve equations under two conditions were established. Results Both under the conditions of artificial and natural degradation, STR/AMEL value had a negative relationship with the degradation time. The relationship between STR/AMEL and degradation time can be well simulated by the cubic function. R2 was over 0.99 under controlled degradation condition and over 0.86 under natural degradation condition. Conclusion The STR/AMEL value （Penta E/AMEL, Penta D/AMEL, FGA/AMEL） is negatively related with the DNA degradation degree, which follows mathematical regression models strictly, and it might be applied to evaluate the DNA degradation degree.

Key words: forensic genetics, DNA degradation, necrotic, short tandem repeat, peak area

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