法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (2): 123-125.DOI: 10.3969/j.issn.1004-5619.2016.02.011

• 技术与应用 • 上一篇    下一篇

不同前处理方法提取牙齿DNA的比较

骆继怀1，2，孙红兵1，2，杨  鑫1，2，张子龙2，王  涛3   

  1. （1. 兰州市公安局 刑事科学技术研究所，甘肃 兰州 730030； 2． 甘肃省证据科学技术研究与应用重点实验室，甘肃 兰州 730070； 3. 甘肃省公安厅，甘肃 兰州 730030）
  • 发布日期:2016-04-25 出版日期:2016-04-28
  • 通讯作者: 孙红兵，男，主任法医师，教授，主要从事法医损伤学、法医物证学研究；E-mail：sunhb.good@163.com
  • 作者简介:骆继怀（1983—），男，硕士研究生，主检法医师，主要从事法医物证鉴定及法医分子生物学研究；E-mail：lanzhou DNA110@126.com
  • 基金资助:

    国家自然科学基金资助项目（81302622）；甘肃省青年科技基金计划资助项目（1308RJYA059）；甘肃省自然科学基金资助项目（145RJZA038）；甘肃省公安厅技术研究计划项目（2016JSYJ01）

Comparison of Different Pretreatment Methods for DNA Extraction from Teeth

LUO JI-HUAI1,2, SUN HONG-BING1,2, YANG XIN1,2, ZHANG ZI-LONG2, WANG TAO3   

  1. （1. Institute of Criminal Science and Technology, Lanzhou Public Security Bureau, Lanzhou 730030, China; 2. Key Laboratory of Evidence of Science and Technology Research and Application of Gansu Province, Lanzhou 730070, China; 3. Gansu Provincial Public Security Department, Lanzhou 730030, China）
  • Online:2016-04-25 Published:2016-04-28

摘要： 目的 对三种不同前处理方法提取的牙齿DNA浓度进行比较，建立一种操作简便、经济适用、浓度较高的牙齿DNA提取的前处理方法。 方法 选择源自7具尸体的共21颗磨牙，每具尸体的3颗磨牙随机按牙屑法、球磨法、液氮研磨法进行前处理，并分别称取50 mg，采用AutoMate ExpressTM法医DNA提取系统提取DNA，对三种方法提取的DNA浓度和STR分型结果进行比较。 结果 牙屑法、球磨法和液氮研磨法提取的DNA质量浓度分别为0.055 6~1.989 1、0.036 6~1.175 6和0.037 8~1.249 0 ng/μL，牙屑法提取的DNA质量浓度较高（P＜0.05），且STR分型成功率高。 结论 牙屑法结合AutoMate ExpressTM法医DNA提取系统是提取牙齿DNA的一种切实可行的方法，可应用于法医学鉴定实践中。

关键词: 法医遗传学, DNA, 牙, 提取法, 前处理

Abstract: Objective To compare the concentration of teeth DNA extracted by three different pretreatment methods and to explore a simple, economical and practical pretreatment method with high concentration of extracted DNA from teeth. Methods A total number of 21 molars were collected from 7 corpses. The pretreatment of 3 molars from each individual was randomly performed by tooth crumb method, ball-milling method and liquid nitrogen milling method and 50 mg tooth crumb was weight and DNA was extracted by AutoMate ExpressTM forensic DNA extraction system. Subsequently, the concentration of DNA and corresponding STR genotyping of three methods were compared. Results The DNA concentration extracted by tooth crumb method, ball-milling method and liquid nitrogen milling method was 0.055 6-1.989 1 ng/μL, 0.036 6-1.175 6 ng/μL and 0.037 8-1.249 0 ng/μL, respectively. The DNA concentration obtained by tooth crumb method was higher （P＜0.05） and the success rate of STR genotyping was high. Conclusion Combined with AutoMate ExpressTM forensic DNA extraction system, tooth crumb method is an efficient and feasible method to extract DNA from teeth, which can be applied in forensic practice.

Key words: forensic genetics, DNA, tooth, extraction, pretreatment

中图分类号: