法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (4): 269-272.DOI: 10.3969/j.issn.1004-5619.2016.04.009

• 技术与应用 • 上一篇    下一篇

南京汉族人群24个Y-STR基因座的遗传多态性

孙大鹏，闵涯邻，连昌舟，俞卫东   

  1. （南京市公安局，江苏 南京 210012）
  • 发布日期:2016-08-25 出版日期:2016-08-28
  • 作者简介:孙大鹏（1980—），男，主检法医师，主要从事法医DNA检验和鉴定；E-mail：9397759@qq.com

Genetic Polymorphisms of 24 Y-STR Loci in Nanjing Han Population

SUN DA-PENG, MIN YA-LIN, LIAN CHANG-ZHOU, YU WEI-DONG   

  1. （Nanjing Public Security Bureau, Nanjing 210012, China）
  • Online:2016-08-25 Published:2016-08-28

摘要： 目的 调查DYS391等24个Y-STR基因座在南京汉族人群的遗传多态性，考察其在法医学中的应用价值。 方法 应用AGCU Y-PLUS（24） PCR试剂盒对南京580名汉族无关男性个体进行Y-STR基因座扩增检测分型，用软件计算24个基因座的基因频率等群体遗传学参数，并与湖北、辽宁、广东、北京、成都汉族人群数据进行比较。 结果 南京580名汉族无关男性个体在24个Y-STR基因座共发现580种单倍型，各基因座的基因多样性（GD）为0.294 6~0.939 8，单倍型多样性（HD）为0.983 7。六地人群GD值的差异有统计学意义。 结论 DYS391等24个Y-STR基因座在南京地区有法医学价值，可用于案件检验及家系排查。

关键词: 法医遗传学, 多态现象, 遗传, 短串联重复序列, Y染色体, 汉族

Abstract: Abstract： Objective To investigate the genetic polymorphism of DYS391 and other 23 Y-STR loci and to explore its application value in forensic science. Methods Y-STRs loci of 580 unrelated Han males in Nanjing were amplified using AGCU Y-PLUS PCR （24） kit. The genetic parameters of 24 Y-STR loci such as gene frequency were calculated by software, and compared with the data of Hubei, Liao- ning, Guangdong, Beijing and Chengdu Han population. Results Total 580 haplotypes were detected among 24 Y-STR loci in 580 unrelated Han males in Nanjing. The genetic diversity （GD） of each locus was from 0.294 6 to 0.939 8, and the haplotypes diversity （HD） was 0.983 7. There was a significant difference between the GD of 6 areas. Conclusion The 24 Y-STR loci such as DYS391 in Nanjing Han population have an application value in forensic science. They can also be used for cases testing and pedigree investigation.

Key words: forensic genetics, polymorphism, genetic, tandem repeat sequences, Y chromosome, Han nationality

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