法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (2): 120-124.DOI: 10.3969/j.issn.1004-5619.2017.02.003

• 论著 • 上一篇    下一篇

大鼠肌肉挥发性有机化合物变化规律与死亡时间的关系

刘蓓蓓，夏志远，马锦琦，李  朴，吕  坪，周海梅   

  1. （河南科技大学法医学院，河南 洛阳 471003）
  • 发布日期:2017-04-25 出版日期:2017-04-28
  • 通讯作者: 周海梅，女，教授，主要从事法医毒物分析教学与研究；E-mail：lyzhouhm@haust.edu.cn
  • 作者简介:刘蓓蓓（1989—），女，硕士研究生，主要从事法医毒物分析的研究；E-mail：legal-medicine@qq.com
  • 基金资助:

    河南省教育厅重点科研基金资助项目（16A150030）；河南科技大学研究生创新基金资助项目（CXJJ-ZR22）

Relationship between the Change Rules of Volatile Organic Compounds in Rat Muscle and Postmortem Interval

LIU BEI-BEI, XIA ZHI-YUAN, MA JIN-QI, LI PU, L?譈 PING, ZHOU HAI-MEI   

  1. （School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, China）
  • Online:2017-04-25 Published:2017-04-28

被引次数

2

摘要： 目的 探究大鼠死后肌肉挥发性有机化合物（volatile organic compound，VOC）的变化规律与死亡时间（postmortem interval，PMI）的关系。 方法 健康大鼠120只，随机分成12组，每组10只，以颈椎脱臼方式处死后保存在（25±1） ℃环境中，在死后即刻（0 d）、0.5、1、2、3、4、5、6、7、8、9、10 d共12个时间点分别取一组大鼠肌肉，采用顶空固相微萃取气质联用法收集、检测、分析大鼠肌肉VOC。 结果 共鉴定出15种VOC，其中芳香类9种、含硫类3种、脂肪酸类2种、杂环类1种。随PMI延长，VOC种类总数呈增加趋势，第1天没有检测到VOC，第2天检出3种，第3天检出9种，第4天检出11种，5~7 d检出14种，8~10 d检出15种。不同VOC峰面积与PMI存在一定的相关性（校准R2=0.15~0.96），15种VOC总峰面积（y）与PMI（x）相关：2~5 d，回归方程为y=-17.05 x2+164.36 x-246.36（校准R2=0.96）；6~10 d回归方程为y=2.24 x+101.13（校准R2=0.97）。 结论 肌肉VOC变化规律有助于PMI的推断。

关键词: 法医病理学, 挥发性有机化合物, 气相色谱-质谱法, 固相微萃取, 死亡时间, 肌, 大鼠

Abstract: Objective To explore the relationship between the change rules of volatile organic compounds （VOCs） in rat muscle and postmortem interval （PMI）. Methods A total of 120 healthy rats were divided randomly into 12 groups （10 for each group）. After the rats were sacrificed by cervical dislocation, the bodies were kept at （25±1） ℃. Rat muscle samples were separately obtained at 12 PMI points, including 0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 d. The VOCs in rat muscles were collected, detected and analyzed by headspace solid-phase microextraction （HS-SPME） coupled to gas chromatography-mass spectrometer （GC-MS）. Results In total, 15 species of VOCs were identified, including 9 aromatic compounds, 3 sulfur compounds, 2 aliphatic acids and 1 heterocyclic compound. The species of VOCs increased with PMI： no species were detected within 1 day, 3 species were detected on day 2, 9 on day 3, 11 on day 4, 14 from day 5 to 7, and 15 from day 8 to 10. Total peak area of 15 species of VOCs was significantly correlated to PMI （adjusted R2=0.15-0.96）： the regression function was y=-17.05 x2+ 164.36 x-246.36 （adjusted R2=0.96） from day 2 to 5, and y=2.24 x+101.13 （adjusted R2=0.97） from day 6 to 10. Conclusion The change rules of VOCs in rat muscle are helpful for PMI estimation.

Key words: forensic pathology, volatile organic compounds, gas chromatography-mass spectrometry, solid phase microextraction, postmortem interval, muscles, rats

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