法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (3): 225-231.DOI: 10.3969/j.issn.1004-5619.2017.03.002

• 论著 • 上一篇    下一篇

HIF-1α、VEGF-A在心律失常大鼠心肌组织中的变化

张  圆1，曹志鹏1，毛瑞明1，2，杜仲波1，米  丽1，3，雒心怡1，田美慧1，朱宝利1   

  1. （1. 中国医科大学法医学院，辽宁 沈阳 110122； 2. 辽宁省公安厅，辽宁 沈阳 110032； 3. 河北北方学院，河北 张家口 075000）
  • 发布日期:2017-06-25 出版日期:2017-06-28
  • 通讯作者: 朱宝利，男，教授，博士研究生导师，主要从事法医病理学教学、研究及鉴定；E-mail：zhu1127@hotmail.com
  • 作者简介:张圆（1988—），女，硕士研究生，主要从事法医病理学研究；E-mail：zhangyuan0917@qq.com
  • 基金资助:

    国家自然科学基金资助项目（81273343）

The Changes of HIF-1α and VEGF-A in Myocardial Tissue of Rats with Arrhythmias

ZHANG YUAN1, CAO ZHI-PENG1, MAO RUI-MING1,2, DU ZHONG-BO1, MI LI1, LUO XIN-YI1, TIAN MEI-HUI1, ZHU BAO-LI1   

  1. （1. School of Forensic Medicine, China Medical University, Shenyang 110122, China; 2. Public Security Department of Liaoning Provincial, Shenyang 110032, China; 3. HeBei North University, Zhangjiakou 075000, China）
  • Online:2017-06-25 Published:2017-06-28

摘要： 目的 观察大鼠心律失常后缺氧诱导因子-1α（hypoxia inducible factor-1α，HIF-1α）和心肌血管内皮生长因子-A（vascular endothelial growth factor-A，VEGF-A）的表达变化，探讨心律失常与冠状动脉供血不足引起的急性心肌缺血中两指标表达规律的不同。 方法 利用CaCl2诱导大鼠心律失常，应用免疫组织化学染色、Western印迹法和实时荧光定量PCR检测大鼠心律失常后6 h内HIF-1α和VEGF-A的表达及变化情况。 结果 心律失常致死的大鼠心肌组织中HIF-1α及VEGF-A呈弥漫性表达，在心律失常发生的早期两者表达均呈增加随后下降；心律失常发生时即产生广泛的心肌缺血且范围不随时间增大。 结论 HIF-1α和VEGF-A在心律失常大鼠心肌组织中均有表达，可为致死性心律失常和冠状动脉供血不足引起的急性心肌缺血的鉴别诊断提供依据。

关键词: 法医病理学, 心律失常, 心性, 心肌, 缺氧诱导因子1, 血管内皮生长因子-A, 大鼠

Abstract: Objective To observe the expression changes of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor-A （VEGF-A） in rats with arrhythmias, and to explore the differences of the expression pattern in the two indicators of acute myocardial ischemia caused by arrhythmias and coronary insufficiency. Methods The arrhythmia was induced by CaCl2, and the expression changes of HIF-1α and VEGF-A were detected by immunohistochemistry, Western blotting and real-time PCR within 6 h after the arrhythmia in rats. Results The expression of HIF-1α and VEGF-A showed diffuse in the myocardial tissue of rats died from arrhythmias. Both of them increased in the early arrhythmia, then decreased. Extensive myocardial ischemia happened at the beginning of arrhythmia occurrence and its range didn’t expand with time. Conclusion The expressions of HIF-1α and VEGF-A in myocardium of the rats with arrhythmia can provide evidence for the differential diagnosis of acute myocardial ischemia caused by fatal arrhythmia and coronary insufficiency.

Key words: forensic pathology, arrhythmias, cardiac, myocardium, hypoxia inducible factor 1, vascular endothelial growth factor-A, rats

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