法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (6): 615-618.DOI: 10.3969/j.issn.1004-5619.2017.06.010

• 技术与应用 • 上一篇    下一篇

应用InnoTyper?誖21试剂盒进行毛干检验

李甫1， 张敏1，王颖希2，水晶晶1，彦萌1，靳小攀3，朱晓君2   

  1. （1. 北京市东城区公安司法鉴定中心，北京 100061； 2. 北京市公安司法鉴定中心，北京 100192； 3. 北京瑞源文德科技有限公司，北京 100176）
  • 发布日期:2017-12-25 出版日期:2017-12-28
  • 通讯作者: 朱晓君，女，主任法医师，主要从事法医物证学研究；E-mail：zhzhzhuxiaojun@sohu.com
  • 作者简介:李甫（1982—），男，主检法医师，主要从事法医物证学研究；E-mail：13911779804@163.com

Determination of Hair Shafts by InnoTyper?誖21 Kit

LI FU1, ZHANG MIN1, WANG YING-XI2, SHUI JING-JING1, YAN MENG1, JIN XIAO-PAN3, ZHU XIAO-JUN2   

  1. （1. Center of Forensic Judicial Appraisal, Dongcheng District Public Security Bureau of Beijing City, Beijing 100061, China; 2. Center of Forensic Judicial Appraisal, Beijing Public Security Bureau, Beijing 100192, China; 3. Ruiyuan Wende Technologies, Ltd., Beijing 100176, China）
  • Online:2017-12-25 Published:2017-12-28

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摘要： 目的 探讨InnoTyper?誖21试剂盒在法医学实践中的应用价值。 方法 收集8名无关个体的毛发及唾液样本，采用AutoMate ExpressTM自动化法医DNA提取系统提取模板DNA，分别采用InnoTyper?誖21和AmpF?詛STRTM IdentifilerTM Plus试剂盒进行扩增，并对检验结果进行比较。 结果 采用InnoTyper?誖21试剂盒扩增时，唾液样本均可检出完整特异性分型，无毛囊毛干分型图谱峰值为57~1 219 RFU，有时可见等位基因缺失；采用AmpF?詛STRTM IdentifilerTM Plus试剂盒扩增时，唾液样本均可检出完整特异性分型，无毛囊毛干均未检出特异性片段。 结论 InnoTyper?誖21试剂盒在无毛囊毛干的检案中具有一定应用价值。

关键词: 法医遗传学, 插入/缺失, 毛干, 个体识别, InnoTyper?誖21试剂盒

Abstract: Objective To explore the application value of InnoTyper?誖21 kit in forensic practice. Methods Samples of hair shafts and saliva were collected from 8 unrelated individuals. Template DNA was extracted by AutoMate ExpressTM forensic DNA automatic extraction system. DNA was amplified by InnoTyper?誖21 kit and AmpF?詛STRTM IdentifilerTM Plus kit, respectively, and then the results were compared. Results After the amplification by InnoTyper?誖21 kit, complete specific genotyping could be detected from the saliva samples, and the peak value of genotyping profiles of hair shafts without sheath cells was 57-1 219 RFU. Allelic gene deletion could be found sometimes. When amplified by AmpF?詛STRTM IdentifilerTM Plus kit, complete specific genotyping could be detected from the saliva samples, and the specific fragment was not detected in hair shafts without sheath cells. Conclusion The InnoTyper?誖21 kit has certain application value in the cases of hair shafts without sheath cells.

Key words: forensic genetics, insertion/deletion, hair shaft, personal identification, InnoTyper?誖21 kit

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