法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (2): 154-156,160.DOI: 10.3969/j.issn.1004-5619.2018.02.010

• 技术与应用 • 上一篇    下一篇

EX16+10Y试剂盒检测新疆维吾尔族人群的效能

毕  钢1，张  晨1，董  研1，焦海涛2，董  雷3，周怀谷1   

  1. 1. 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083； 2. 无锡中德美联生物技术有限公司，江苏 无锡 214174； 3. 乌鲁木齐市公安局刑事科学技术研究所，新疆 乌鲁木齐 830063
  • 发布日期:2018-04-25 出版日期:2018-04-28
  • 通讯作者: 周怀谷，男，博士，主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：hgzhou803@hotmail.com
  • 作者简介:毕钢（1962—），男，博士，副主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：zhongbi2003@qq.com
  • 基金资助:
    上海市科研计划资助项目（13JG0500200）

Efficiency Analysis of EX16+10Y Kit on Detection of the Uygur Population in Xinjiang Province

BI Gang1, ZHANG Chen1, DONG Yan1, JIAO Hai-tao2, DONG Lei3, ZHOU Huai-gu1   

  1. 1. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. AGCU ScienTech Incorporation, Wuxi 214174, China; 3. Institute of Criminal Science and Technology, Urumqi Public Security Bureau, Urumqi 830063, China
  • Online:2018-04-25 Published:2018-04-28

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摘要： 目的 检验EX16+10Y试剂盒在新疆维吾尔族人群中的法医学检测效能。 方法 采集4 620个新疆地区维吾尔族男性个体血样，用EX16+10Y试剂盒进行扩增，用3130xl基因分析仪对扩增产物进行分型。 结果 获得4 620个新疆维吾尔族男性个体的15个常染色体STR基因座和10个Y染色体STR基因座的完整分型图谱。15个常染色体STR基因座分布均符合Hardy-Weinberg平衡，STR基因座的杂合度为0.637~0.838，多态信息含量为0.580~0.860，个体识别率为0.811~0.978。10个Y染色体STR基因座有766种单倍型。 结论 EX16+10Y试剂盒检测结果准确可靠，可用于实际工作中同时完成个体识别和男性家系排查。

关键词: 法医遗传学, Y染色体, 短串联重复序列, EX16+10Y试剂盒, 新疆, 维吾尔族

Abstract: Objective To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province. Methods The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130xl genetic analyzer. Results The genotyping graphs of 15 autosomal STR loci and 10 Y-chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely. The genotype distribution of 15 autosomal STR loci was consistent with Hardy-Weinberg equilibrium. The heterozygosity, polymorphism information content and discrimination power of STR loci were 0.637-0.838, 0.580-0.860 and 0.811-0.978, respectively. There were 766 haplotypes in 10 Y -chromosomal STR loci. Conclusion The test results of EX16+10Y kit is accurate and trustworthy, which can simultaneously be used for the individual identification and the screening of paternal pedigree in practical work.

Key words: forensic genetics, Y chromosome, short tandem repeat, EX16+10Y kit, Xinjiang, Uygur nationality