法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (2): 161-164.DOI: 10.3969/j.issn.1004-5619.2018.02.012

• 技术与应用 • 上一篇    下一篇

RapidHITTM 200系统在法医学中的应用

孙  帅1，田雨苗2，汤继明3，张庆霞4，胡玉龙5，王鹏飞6，薛卢艳4，刘  力4，唐  晖7   

  1. 1. 山西医科大学晋祠学院，山西 太原 030025； 2. 山西医科大学第一临床医学院，山西 太原 030001； 3. 阿克苏地区公安局，新疆 阿克苏 843000； 4. 北京市公安司法鉴定中心，北京 100192； 5. 鄂尔多斯市公安局禁毒支队，内蒙古 鄂尔多斯 017000； 6. 包头市土默特右旗公安局，内蒙古 包头 014100； 7. 浙江迪安鉴定科学研究院，浙江 杭州 310030
  • 发布日期:2018-04-25 出版日期:2018-04-28
  • 通讯作者: 唐晖，女，博士，主要从事法医物证学研究；E-mail：huihuitangno1@163.com
  • 作者简介:孙帅（1989—），男，硕士，主要从事法医物证学研究；E-mail：ss0030@163.com

Application of RapidHITTM 200 System in Forensic Medicine

SUN Shuai1, TIAN Yu-miao2, TANG Ji-ming3, ZHANG Qing-xia4, HU Yu-long5, WANG Peng-fei6, XUE Lu-yan4, LIU Li4, TANG Hui7   

  1. 1. Jinci College of Shanxi Medical University, Taiyuan 030025, China; 2. First Clinical Medical College of Shanxi Medical University, Taiyuan 030001, China; 3. Aksu District Public Security Bureau, Aksu 843000, China; 4. Forensic Judicial Appraisal Center of Beijing Public Security Bureau, Beijing 100192, China; 5. Counter-narcotics Detachment of Ordos Public Security Bureau, Ordos 017000, China; 6. Tumoteyou Branch of Baotou Public Security Bureau, Baotou 014100, China; 7. Institute of Science and Identification, DIAN, Hangzhou 310030, China
  • Online:2018-04-25 Published:2018-04-28

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摘要： 目的 验证RapidHITTM 200系统对四类法医学常见检材的检测效能，以及两次重复检测运行中模板、模板DNA和PCR产物的再利用效能。 方法 将口腔拭子经RapidHITTM 200系统先后进行两次检验，并针对系统中产生的模板DNA和PCR产物进行首次、再次检测。将四类法医学常见检材经RapidHITTM 200系统检测，并针对系统运行中产生的模板、模板DNA和PCR产物进行后续检测。 结果 口腔拭子经该系统首次运行后STR基因座可以完全检出，再次检测过程中部分STR基因座有丢失，再次检测峰值较首次明显降低；模板DNA和PCR产物再次检测的平均STR基因座检出率均小于50%，明显低于首次检测结果。该系统对组织与口腔拭子的检测效能优于血拭子和烟蒂，首次运行后的相应检材、模板DNA、PCR产物延续后续实验流程所获得的STR基因座检出率呈递减趋势。 结论 RapidHITTM 200系统对口腔拭子再检测效果好，但经该系统首次运行后的检材、模板DNA、PCR产物以及经该系统再次运行后的模板DNA与PCR产物不应直接用于法医学后续应用及研究。

关键词: 法医遗传学, 短串联重复序列, RapidHITTM 200系统, 一致性检测

Abstract: Objective To validate the analysis capability of RapidHITTM 200 system for four kinds of routine forensic samples and the recyclable capability of template, template DNA and PCR products in the process of twice duplicate detection. Methods The buccal swabs underwent the test twice by RapidHITTM 200 system, and the template DNA and PCR products that arose in the system were also tested for two times. After four kinds of routine forensic samples were detected by RapidHITTM 200 system, the follow-up tests of the template, template DNA and PCR products that arose in the system were performed. Results The STR loci could be detected in the buccal swabs by the system for the first time. However, part of the STR loci lost during the second test. And the peak value obtained in the second test was significantly reduced than the one in the first time. The average STR loci detection rates of the template DNA and PCR products were both less than 50% in the second test, which were significantly reduced than that in the first test. In addition, the analysis capability of the system for the tissues and buccal swabs was better than that for the blood and cigarette butts. Compared with the first test, the STR loci detection rate of the tested items, template DNA and PCR products decreased with the numbers of tests. Conclusion RapidHITTM 200 system is more effective in retesting buccal swabs than other samples, whereas the items, DNA template, PCR products obtained in the first and second time cannot be directly used for the further application and study of forensic medicine.

Key words: forensic genetics, short tandem repeat, RapidHITTM 200 system, consistency checking