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法医学杂志 2018, Vol. 34 Issue (3) :223-227    DOI: 10.12116/j.issn.1004-5619.2018.03.001
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温度对死后大鼠肾组织FTIR光谱特征的影响
王  磊1,2,3,王  琪4,林汉成5,黄  平3,邓恺飞3,罗仪文3,孙其然3,张清华3,王振原5,孙俊红2,托  娅1
1. 上海健康医学院基础医学院,上海 201318; 2. 山西医科大学法医学院,山西 太原 030001; 3. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台,上海 200063; 4. 西安市公安局刑事侦查局技术处,陕西 西安 710038; 5. 西安交通大学医学部法医学院,陕西 西安 710061
Effects of Temperature on FTIR Spectral Characteristics of Renal Tissue in Rats after Death#br#
WANG Lei1,2,3, WANG Qi4, LIN Han-cheng5, HUANG Ping3, DENG Kai-fei3, LUO Yi-wen3, SUN Qi-ran3, ZHANG Qing-hua3, WANG Zhen-yuan5, SUN Jun-hong2, TUO Ya1
1. School of Basic Medical Science, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China; 2. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 3. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China; 4. Technology Division of Criminal Investigation Department, Xi’an Public Security Bureau, Xi’an 710038, China; 5. College of Forensic Science, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China`

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摘要 目的 分析大鼠死后肾组织在不同温度下的傅里叶变换红外(Fourier transform infrared,FTIR)光谱数据,探究温度对死后大鼠肾组织FTIR光谱特征的影响。 方法 大鼠颈椎脱臼处死后置于4 ℃、20 ℃和30 ℃环境中,于不同时间点提取大鼠肾组织,采集FTIR光谱数据,并利用数据挖掘技术对数据进行分析。 结果 4 ℃、20 ℃和30 ℃环境组中部分时间点样本主成分分析结果具有明显聚类趋势。分别利用三个温度组光谱数据建立偏最小二乘(partial least square,PLS)回归模型,结果20 ℃和30 ℃组数据的PLS回归模型性能均优于4 ℃组,20 ℃组模型稳定性优于30 ℃组。 结论 大鼠死后肾组织在不同温度下的FTIR光谱特征具有差异,温度对FTIR光谱PLS回归模型性能具有重要影响,因此在利用光谱法推断死亡时间的研究中需要考虑温度对模型的影响,以提高死亡时间推断的准确性。
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王磊
王琪
林汉成
关键词法医病理学;谱学   傅里叶变换红外;主成分分析;偏最小二乘法;死亡时间;温度;肾;大鼠     
Abstract: Objective To analyse the Fourier transform infrared (FTIR) spectral data of renal tissue at different temperatures in rats after death, and to explore the effects of temperature on the FTIR spectral characteristics of renal tissue. Methods The rats were sacrificed by cervical dislocation and placed at 4 ℃, 20 ℃ and 30 ℃. The FTIR spectral data of renal tissue were collected at different time points and analysed by data mining method. Results The principal component analysis (PCA) results showed that there were significant trends of clustering in the samples of partial time point at 4 ℃, 20 ℃ and 30 ℃. Partial least square (PLS) regression models were established with the spectral data at three temperature groups. The performance of PLS regression models in 20 ℃ and 30 ℃ groups were more superior than that in 4 ℃ group, and the stability of the model in 20 ℃ group was better than that in 30 ℃ group. Conclusion There are differences in the FTIR spectral characteristics of renal tissue of rats after death at different temperatures. Temperature has a major impact on the performance of FTIR spectral PLS regression model. Therefore, in order to improve the accuracy of postmortem interval estimation, the effects of temperature on the model should be considered in the related study by spectral method.
Keywordsforensic pathology   spectroscopy, Fourier transform infrared   principal component analysis   partial least square method   postmortem interval   temperature   kidney   rats     
Fund:国家自然科学基金资助项目(81601645,81571852, 81671869);“十三五”国家重点研发计划资助项目(2016YFC08 00702);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)
Corresponding Authors: 托娅,女,博士,主要从事法医病理学研究;E-mail:tuoy@sumhs.edu.cn 孙俊红,男,博士,教授,主要从事损伤病理学和猝死病理学研究;E-mail:sunjunhong146@163.com   
About author: 王磊(1990—),男,硕士研究生,主要从事法医病理学研究;E-mail:leilei1219063@126.com
引用本文:   
王磊,王琪,林汉成,等.温度对死后大鼠肾组织FTIR光谱特征的影响[J]  法医学杂志, 2018,34(3): 223-227
WANG Lei, WANG Qi, LIN Han-cheng,et al.Effects of Temperature on FTIR Spectral Characteristics of Renal Tissue in Rats after Death#br#[J]  法医学杂志, 2018,34(3): 223-227
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