法医学杂志 ›› 2016, Vol. 32 ›› Issue (1): 21-25.DOI: 10.3969/j.issn.1004-5619.2016.01.005

• 论著 • 上一篇    下一篇

猪肋软骨和肋骨的ATR-FTIR光谱变化与死亡时间的关系

姚  尧1,2,王  琪1,荆小莉2,李  冰1,张胤鸣1,王志军1,李成志1,林汉成1,张  吉1,黄  平3,王振原1   

  1. (1. 西安交通大学法医学院,陕西 西安 710061; 2. 西安市公安局高新分局,陕西 西安 710065; 3. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室,上海 200063)
  • 发布日期:2016-02-25 出版日期:2016-02-28
  • 通讯作者: 黄平,男,博士,研究员,主任法医师,主要从事法医病理学研究及鉴定;E-mail:huangp@ssfjd.cn 王振原,男,教授,博士研究生导师,主要从事法医病理学研究及鉴定;E-mail:wzy218@mail.xjtu.edu.cn
  • 作者简介:姚尧(1985—),男,硕士研究生,主要从事法医病理学研究及鉴定;E-mail:yaoxiangshuai@qq.com
  • 基金资助:

    国家自然科学基金资助项目(81273339、81273335)

Relationship between PMI and ATR-FTIR Spectral Changes in Swine Costal Cartilages and Ribs

YAO YAO1,2, WANG QI1, JING XIAO-LI2, LI BING1, ZHANG YIN-MING1, WANG ZHI-JUN1, LI CHENG-ZHI1, LIN HAN-CHENG1, ZHANG JI1, HUANG PING3, WANG ZHEN-YUAN1     

  1. (1. School of Forensic Science and Medicine, Xi’an Jiaotong University, Xi’an 710061, China; 2. Gaoxin Branch of Xi’an Public Security Bureau, Xi’an 710065, China; 3. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China)
  • Online:2016-02-25 Published:2016-02-28

摘要: 目的 应用衰减全反射傅里叶变换红外(attenuated total reflection-Fourier transform infrared,ATR-FTIR)光谱技术分析长白猪死后肋软骨和肋骨组织随死亡时间推移的化学降解过程,为死后较长时间段内推断死亡时间提供可行的新途径和方法。 方法 猪放血处死后取肋软骨和肋骨离体置于20 ℃环境,后每72 h提取部分组织样本,进行ATR-FTIR光谱分析,并与对应死亡时间点进行相关性分析。 结果 随死亡时间的延长,肋软骨与肋骨FTIR的主要吸收峰峰位没有发生明显变化,而其部分峰强比出现了不同时序性的变化趋势,并与死亡时间存在良好的相关性,肋软骨较肋骨组织有更好的时序性。 结论 应用ATR-FTIR光谱技术发现死后猪肋软骨和肋骨组织光谱学变化存在一定的规律性,有望成为基于光谱学推断死亡时间的一种新途径。

关键词: 法医病理学, 谱学, 傅里叶变换红外, 衰减全反射, 肋骨, 肋软骨, 死亡时间,

Abstract: Objective To analyze postmortem chemical changes in Landrace costal cartilages and ribs using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and to provide a novel technique for estimation of postmortem interval (PMI). Methods The swines were sacrificed by hemorrhage and their costal cartilages and ribs were kept in 20 ℃. The chemical analysis of the costal cartilages and ribs were performed using ATR-FTIR every 72 h. The correlation between the certain spectral parameters and PMI was also analyzed. The time-dependent changes of costal cartilages were more significant than ribs. Results There were no obvious changes for the main absorbance bands position, and some absorbance band ratios showed time-dependent changes and significant correlations with the PMI. Conclusion ATR-FTIR has the ability to analyze postmortem chemical changes of the swine costal cartilages and ribs, and it can be a new method to estimate PMI based on spectroscopy.

Key words:  forensic pathology, spectroscopy, Fourier transform infrared, attenuated total reflection, ribs, costal cartilage, postmortem interval, swine

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