法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (5): 321-323.DOI: 10.3969/j.issn.1004-5619.2011.05.001

• 论著 •    下一篇

家兔死后心肌酰胺A带的傅里叶变换红外光谱图像分析

张海东1，杨天潼1，郑  娜2，孙婷怡2，刘  良1   

  1. （1. 中国政法大学 证据科学教育部重点实验室，北京 100040； 2. 华中科技大学 同济医学院法医学系，湖北 武汉 430030）
  • 发布日期:2011-10-25 出版日期:2011-10-28
  • 作者简介:张海东（1969—），男，山东乐陵人，副教授，主要从事法医病理学教学、科研及鉴定工作；E-mail：zhd_zzw@126.com
  • 基金资助:

    国家自然科学基金青年科学基金项目（81001349）

Analysis of the Changes of Amide A in Rabbit Heart after Death by FTIR Spectroscopy

ZHANG HAI-DONG1, YANG TIAN-TONG1, ZHENG NA2, SUN TING-YI2, LIU LIANG1   

  1. （1. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100040, China; 2. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China）
  • Online:2011-10-25 Published:2011-10-28

被引次数

5

摘要： 目的 应用傅里叶变换红外光谱成像系统研究家兔心肌酰胺A带变化与死亡时间的关系。 方法 将32只家兔处死后取出心脏，20 ℃保存，于48 h内不同时间点取样并制作切片。用傅里叶变换红外光谱成像系统绘制酰胺A带图像，分析研究死后酰胺A带阳性与阴性面积比的变化规律。 结果 48 h内随死亡时间（x）的延长，酰胺A带的阳性与阴性面积比（y）逐渐下降，二次曲线回归方程为y=0.001 x2-0.038 x+0.747（R2=0.940）。 结论 酰胺A带的阳性与阴性面积比与死亡时间有明显的相关性，可望用于推断早期死亡时间。

关键词: 法医病理学, 谱学,傅里叶变换红外, 心肌, 酰胺A带, 死亡时间, 兔

Abstract: Objective To explore the relationship of changes of amide A in rabbit heart and the postmortem interval（PMI） by FTIR spectroscopy technique. Methods Thirty-two rabbits were sacrificed and the hearts were sampled at 20 ℃ within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system. Results The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios（y） of amide A（positive and negative area） and PMI（x）. The regression equation was y=0.001 x2-0.038 x+0.747（R2=0.940）. Conclusion The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.

Key words: forensic pathology, spectroscopy, Fourier transform infrared, myocardium, amide A, postmortem interval, rabbits

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