›› 1998, Vol. 0 ›› Issue (1): 14-15+1.

• 论文 • Previous Articles     Next Articles

Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes

ZHENG XIUFEN, YE JIAN, NI JINTANG, LI BOLING(INSTITUTE OFFORENSIC SCIENCE, P.R.CHINA, BEJING 100038)   

  • Online:1998-02-25 Published:1998-02-28

Abstract: The pulPose of this study is to detect small amount ofbiological materials using the method of MVR-PCR atDIS8 locus with HRP-labeled MS32 oligonucleotidesprobe. The results showed that the MVR-PCR patterns,obtained from trace blood stain (equal to 5 ul blood),trace saliva stain (equal to 4 ul saliva) or a hair root, canbe read clearly and easily. The sensitivity of the methodis 1 ng DNA. The applications of the method in personalidentification were discussed.

Key words: D1S8 locus, MVR-PCR, HRP-labeled probe