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    28 February 1998, Volume 14 Issue 1 Previous Issue    Next Issue

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    Encephalic morphologic changes due to delayed traumatic intracerebral hematoma
    ZHANG GUOHUA. BE DEWEN;LI QINGSHENG;CHENHUAFOR;SHAN YAMING (DEPARTMENT OF FORENSICPATHOLOGY FACULTY OF FORENSIC MEDICINE. CHINAMEDICAL UNIVERSITY;SHENYAN 000)
    1998, 0(1): 1-3+61. 
    Abstract ( 1881 )  
    Pathological changes of brain tissues due to delayedtraumatic inthecerebral hematoma (DTICH) in 6 caseswere studied and the mechanism was discussed. It wasobserved that DTICH went with old brain contusion,traumatic angionecrosis, vasculitis, calcification, encapsulated old focus, proliferation and reactive hypertrophyin glia. Brain contusions and traumatic vessel lesions areimportant pathological foundations of cerebral hemorrhage and primary causes of DTICH.
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    Head trauma and brain auditory evoked potentials
    FAN LIHUA;SUN HONG;ZHU GUANGYOU;SHEN YAN(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R. CHINA SHANGHAI. 000)
    1998, 0(1): 4-5+3. 
    Abstract ( 2064 )  
    The study investigated brain auditory evoked potentials(BAEP) in patients complaining auditory disturbancesafter head trauma. The results showed that the incidenceof hearing loses was 57.5% and the rate of abnormalityof BAEP is 33.75%. The types of the abnormalities ofBAEP varied and were related with the position andseventy of the head trauma. The types of the abnormalities manifested as loses of Ⅰ-Ⅱ waves, prolongstionof V latency and Ⅰ-Ⅴ interlatency.
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    Decomposition kinetics of methamidophos in preserved blood of rabbit
    ZHUO XIARYI;SHEN BAOHUA;SUN YAJUAN (INSTITUTE OFFORENSIC SCIENCES;MINISTRY OF JUSTICE. P.R.CHINA;SHANGHAI 000)
    1998, 0(1): 6-7+61. 
    Abstract ( 1937 )  
    Decomposition kinetics of methmidophos in preservedrabbit blood poisoned by methamidophos was studied.The blood was preserved in a room (temperature 12-15℃) and in a refrigerator (temperature 2-5℃). Theconcentration of methamidophos in preserved blood wasdetermined by GC/FPD. The results showed that thedecomposition of methamidophos in preserved blood ofrabbits was fast for the first three days and got slow withthe time prolonged. The decomposition speed ofmethamidophos in room was higher than in refrigerator.
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    Application of VNTR D17S30 locus polymorphism in the paternity test
    FENG MINGLIANG;FENG ZHE LU QING. ZHANGZ-HANG;YANG YING;JI YUEHUA;CHEN RENBIAO (IMMUNO-GENTETICSLABORATORY;SHANGHAI INSTITUTUTE OF BLOOD TRANSFUSION;SHANGHAI BLOOD CENTER;SHANGHAI 000)
    1998, 0(1): 8-9+61. 
    Abstract ( 1987 )  
    A sensitive and rapid PCR-based technique was adoptedto genotype the VNTR D17S30 locus. It was confirmedthrough the genetic analysis of 20 normal families thatthe inheritance of D17S30 locus coincides withMendelian law as simple co-dominant. Retrospective analysis of 100 Paternity cases demonstrated thatD17S30 locus could be used in forensic paternity test inour counts. The exclusion probability estimated fromallele frequencies of D17S30 locus (74.04%) does notdiffer significantly from the observed rate of exclusion(80.00%) in these cases. In all excluded paternity casesthere are two in which the exclusion evidence is solelyprovided by the D17530 locus.
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    Analysis of MDMA and its metabolites in urine by GC and GC/MS
    SHEN MIN;YAN WEIMIN;SHEN BAOHUA;XIANG PING;HUANG ZHONGIE. LIU WEI;BO JUN(INSTITUTE OF FORENSICSCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000)
    1998, 0(1): 10-11+2. 
    Abstract ( 2247 )  
    The purpose of this stUdy is to investigate themetabolism of MDMA in the person and establish themethods for the determination of MDMA and itSmetabolites in urine. MDMA and itS metabolite wereisolated from urine by liquid-liquid extraction afteracidic or enzymatic hydrolysis and were determined byGC/MS(EI、 PCI) and GC/FID. The results showed thatMDMA was metabolized to MDA and HMMA in man.MDA in the urine after MDMA administration wasapproximately 0.10-0.14 that of the parent drug. Thesensitivity limits of methods were 2-50 ng/ml, and therecoveries were greater than 85%(CV<10%). It isconcluded that the methods were simple, quick andaccurate for determining urine concentration of MDMAaddicts. MDA/MDMA Ratio can be used to indicatewhether the parent drug is taken alone or in combinationwith itS active metabolite.Keywrds: MDMA; metabolites; metabolite to parentratio
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    Polymorphism analysis of D6S366 STR locus
    YU CHUNYING;YANG QINGEN;YANG RONGZHI. ZHUCHUANHONG;HU JIAWEI (DEPARTMENT OF FORENSICMEDICINE;TONG JI MEDICAL UNIVERSITY WUHAN 000)
    1998, 0(1): 12-13+6. 
    Abstract ( 1874 )  
    The polymorphic STR locus D6S366 was analyzedusing PCR and PAG electrophoresis and 7 alleles weredetected from 200 unrelated individuals in Wuhan. Theallete distribution of the locus was in accordance withHardy-Weinberg equilibrium. The H, Dp, P, E, and PICof the D6S366 were calculated in this paper.
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    Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes
    ZHENG XIUFEN;YE JIAN;NI JINTANG;LI BOLING(INSTITUTE OFFORENSIC SCIENCE;P.R.CHINA;BEJING 000)
    1998, 0(1): 14-15+1. 
    Abstract ( 2030 )  
    The pulPose of this study is to detect small amount ofbiological materials using the method of MVR-PCR atDIS8 locus with HRP-labeled MS32 oligonucleotidesprobe. The results showed that the MVR-PCR patterns,obtained from trace blood stain (equal to 5 ul blood),trace saliva stain (equal to 4 ul saliva) or a hair root, canbe read clearly and easily. The sensitivity of the methodis 1 ng DNA. The applications of the method in personalidentification were discussed.
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    Study of test method of A, B and H substances in human tissues
    LIU ZHIPING;WU WENQING;ZHOU YUEQIN (DEPARTMENL OFFORENSIC MEDICINE. SHANGHAI MEDICAL UNIVERSITYSHANGHAI 000 )
    1998, 0(1): 16-17+6. 
    Abstract ( 1691 )  
    With the help of the red cell adherence test, absorption-.inhibition test and absorption-elution test, the A, B, Oblood type of different tissues of corpses, whose ABOblood-type was known, was examined. The Substance ofA, B, H type in 16 kinds of tissues of corpses was thesame as in blood stain of corpses. The checked timelimit of A, B, H substance of tissues preserved indifferent temperature was studied. The checked time ofA, B, H substance of the tissues in 4 ℃ was longer thanin room temperature, and the time in the hollow organ(vissera) shorter than that in parenchymatous organ(vissera).
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    Comparative studiy on DNA abstraction of human tissues preseved by two different methods
    XU HONGBIN (DEPARTMENT OF BIOCHEMISLRY WANNANMEDICAL COLLEGE. WUHU 00)
    1998, 0(1): 18-19+6. 
    Abstract ( 1927 )  
    Comparative studies have been made on the effect ofDNA abstraction of different human tissues preserved bydifferent methods. The results showed that differenthuman tissues preserved in 50% alcohol may havesimilar DNA abstraction effect for a relatively long timeas frozen tissues. They have no significant differenceThis preservation method has theadvantage of simplicity, high DNA output and goodeffect, and is suitable for preserving tissues underspecial circumstances.
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    Simultaneous phenotyping of EAP and PGMI by PAGIF
    LIU YAN(LNSLITULE OF FORENSIC SCIENCE;MINISLRY OFJUSTICE;P. R.CHINA;SHANGHAI 000)
    1998, 0(1): 21-24+6. 
    Abstract ( 1955 )  
    The study is to find new genetic markers of simultaneousphenotyping and to raise the determinating power ofisoenzymes. Simultaneous electrophoresis of EAP andPGMI by thin-layer PAGIF combined with reversezymogram developing was applied and clear isoenzymebands was observed on both surface of a gel withoutinterference.The method can do typing of the EAP andPGMl at one gel and the accumulative DP is 0.87 It canbe used in paternity test.
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    Analysis of 3130 cases of mcdicolegal postmortem
    HUANG CHAOXU(FACULTY OF LAW;SHANTOU UNIVERSITYSHANTOU 0)
    1998, 0(1): 22-24+6. 
    Abstract ( 1935 )  
    3130 cases of medicolegal postmortem in Shantou CitybetWeen January 1977 and December 1996 wereanalyzed statistically. The results revealed that the ratioof male to female was 2.3 and most dead were youngand middle age. The number of postmortem hasincreased in resent years. Of 3130 cases, 2829 (92.3%)were caused by mechanical injury, mechanical asphyxia,sudden death and poisoning.As to manners of thedeath. 1226 were related to accidents (39.2%), 776homicide(29.8%), 658 suicide (21.0%). These resultswere compared with other studies reported in domesticand international literature.
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