›› 1999, Vol. 0 ›› Issue (3): 141-143+.

• 论文 • Previous Articles     Next Articles

PCR-based DNA analysis of vWA31A, TH01, F13A01, FESFPS, TPOX and CSF1PO

JIANG BIN, GUO JINGYUAN, LIANG SHANGQIU (DEPARTMENT OF FORENSIC. SCIENCE, THE SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCE, GUANGZHOU 510089, P. R. CHINA)   

  • Online:1999-06-25 Published:1999-06-28

Abstract: The allele and genotype frequencies of 6 tetranucleotide STR loci were investigated in a sample of 132 unrelated individuals from Chinese Han population. The PCR products were analyzed on 6% denaturing PAGE and detected using fluoreseently labeled primers in an automated 377 sequencer(PE). All loci meet Hardy- Weinberg equilibrium. There was no random association of alleles among the 6 loci. The allele frequencies were compared with other population databases. Except locus vWA31A, the observed heterozygosity at other 5 loci was significantly lower than that reported in Caucasian and Black population studies. The calculated DP=0.99999, PE=0.9708, pM=1.059×10~(-5). The allelic frequency data can be used in forensic identification and paternity testing.

Key words: vWA31A, TH01, F13A01, FES/FPS, TPOX, CSF1PO, PCR