Abstract: Cultured human fetal lung Fbs was first used to study the relationship between wound age and the change of cFn synthesized by Fbs in Vitro A sterilized injector was applied to scrap the cultured confluent Fbs and a“wound”was obtained Fbs around the wound was seen to change from a stationary stage to an active stage Using the method of immunochemistry and image analysis system (IAS), cFn synthesized by Fbs around the wound was observed at once, 30min, 1h, 3h and 6h after injury It was found that cFn began to change 1h after injury and increased continually within 6h It suggests that the change of cFn synthesized by Fbs is time-dependent after injury and this approach breaks a new path in the study on estimation of wound age

Key words: wound age, cell-culture, fibroblasts, fibronectin, immunohistochemistry, image analysis system