Application Prospect of MicroRNA in Skin Wound Age Estimation

doi:10.12116/j.issn.1004-5619.2020.400709

Abstract

Abstract:

Wound age estimation is one of the major tasks in forensic practice. However, relatively accurate estimation of the wound age is still a conundrum and research spotlight world-widely. Studies show that microRNAs (miRNAs) are involved in the whole process of the skin wound repair, and miRNAs, as biomarkers, might be used to estimate the time of skin injury owing to their characteristic advantage. This paper summarizes the miRNA fundamental function, properties, current research progress in the estimation of wound age, and its limitations, and put forward prospect of potential application and research based on miRNAs in estimation of wound age.

Key words: forensic pathology, wound age estimation, skin, microRNA, review

CLC Number:

DF795.1

Jian CHENG, Long-long SUO, Lin-lin WANG, Rui ZHAO, Da-wei GUAN. Application Prospect of MicroRNA in Skin Wound Age Estimation[J]. Journal of Forensic Medicine, 2021, 37(6): 841-846.

References 70

1	GRELLNER W， MADEA B. Demands on scientific studies： Vitality of wounds and wound age estimation[J]. Forensic Sci Int，2007，165（2/3）：150-154. doi：10.1016/j.forsciint.2006.05.029.
2	官大威，赵锐，王林林. 法医学损伤时间推断：过去、现在与未来[J].法医学杂志，2019，35（2）：131-135. doi：10.12116/j.issn.1004-5619.2019.02.001.
	GUAN D W， ZHAO R， WANG L L. Forensic injury timing inference： Past， present and future[J]. Fayixue Zazhi，2019，35（2）：131-135.
3	CECCHI R. Estimating wound age： Looking into the future[J]. Int J Legal Med，2010，124（6）：523-536. doi：10.1007/s00414-010-0505-x.
4	YAGI Y， MURASE T， KAGAWA S， et al. Immunohistochemical detection of CD14 and combined assessment with CD32B and CD68 for wound age estimation[J]. Forensic Sci Int，2016，262：113-120. doi：10.1016/j.forsciint.2016.02.031.
5	DU Q， LI N， DANG L， et al.Temporal expression of wound healing-related genes inform wound age estimation in rats after a skeletal muscle contusion： A multivariate statistical model analysis[J]. Int J Legal Med，2020，134（1）：273-282. doi：10.1007/s00414-018-01990-2.
6	MULHOLLAND E J， DUNNE N， MCCARTHY H O. MicroRNA as therapeutic targets for chronic wound healing[J]. Mol Ther Nucleic Acids，2017，8：46-55. doi：10.1016/j.omtn.2017.06.003.
7	MENG Z， ZHOU D， GAO Y， et al. miRNA delivery for skin wound healing[J]. Adv Drug Deliver Rev，2018，129：308-318. doi：10.1016/j.addr.2017.12.011.
8	KONDO T， ISHIDA Y. Molecular pathology of wound healing[J]. Forensic Sci Int，2010，203（1/2/3）：93-98. doi：10.1016/j.forsciint.2010.07.004.
9	LEE R C， FEINBAUM R L， AMBROS V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell，1993，75（5）：843-854. doi：10.1016/0092-8674（93）90529-Y.
10	REINHART B J， SLACK F J， BASSON M， et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans[J]. Nature，2000，403（6772）：901-906. doi：10.1038/35002607.
11	LAGOS-QUINTANA M， RAUHUT R，LENDECKEL W， et al. Identification of novel genes coding for small expressed RNAs[J]. Science，2001，294（5543）：853-858. doi：10.1126/science.1064921.
12	WANG X. Composition of seed sequence is a major determinant of microRNA targeting patterns[J]. Bioinformatics （Oxford， England），2014，30（10）：1377-1383. doi：10.1093/bioinformatics/btu045.
13	BANERJEE J， CHAN Y C， SEN C K. Micro-RNAs in skin and wound healing[J]. Physiol Genomics，2011，43（10）：543-556. doi：10.1152/physiolgenomics.00157.2010.
14	ØROM U A， NIELSEN F C， LUND A H. Micro-RNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation[J]. Mol Cell，2008，30（4）：460-471. doi：10.1016/j.molcel.2008.05.001.
15	TAY Y， ZHANG J， THOMSON A M， et al. Micro-RNAs to Nanog，Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation[J]. Nature，2008，455（7216）：1124-1128. doi：10.1038/nature07299.
16	BARTEL D P. MicroRNAs： Genomics， biogenesis， mechanism， and function[J]. Cell，2004，116（2）：281-297. doi：10.1016/S0092-8674（04）00045-5.
17	YEKTA S， SHIH I， BARTEL D P. MicroRNA-directed cleavage of HOXB8 mRNA[J]. Science，2004，304（5670）：594-596. doi：10.1126/science.1097434.
18	TANG R， LI L， ZHU D， et al. Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus： Evidence for a microRNA hierarchy system[J]. Cell Res，2012，22（3）：504-515. doi：10.1038/cr.2011.137.
19	KOZOMARA A， BIRGAOANU M， GRIFFITHS-JONES S. miRBase： From microRNA sequences to function[J]. Nucleic Acids Res，2019，47（D1）：D155-D162. doi：10.1093/nar/gky1141.
20	GEBERT L F R， MACRAE I J. Regulation of micro-RNA function in animals[J]. Nat Rev Mol Cell Bio，2019，20（1）：21-37. doi：10.1038/s41580-018-0045-7.
21	AMBROS V. The functions of animal microRNAs[J]. Nature，2004，431（7006）：350-355. doi：10.1038/nature02871.
22	BARRIENTOS S， STOJADINOVIC O， GOLINKO M S， et al. Growth factors and cytokines in wound healing[J]. Wound Repair Regen，2008，16（5）：585-601. doi：10.1111/j.1524-475X.2008.00410.x.
23	MAHDAVIAN DELAVARY B， VEER W M VAN DER， EGMOND M VAN， et al. Macrophages in skin injury and repair[J]. Immunobiology，2011，216（7）：753-762. doi：10.1016/j.imbio.2011.01.001.
24	SHAH J M Y， OMAR E， PAI D R， et al. Cellular events and biomarkers of wound healing[J]. Indian J Plast Surg，2012，45（2）：220-228. doi：10.4103/0970-0358.101282.
25	WERNER S， PETERS K G， LONGAKER M T， et al. Large induction of keratinocyte growth factor expression in the dermis during wound healing[J]. Proc Natl Acad Sci USA，1992，89（15）：6896-6900. doi：10.1073/pnas.89.15.6896.
26	PILCHER B K， DUMIN J A， SUDBECK B D， et al. The activity of collagenase-1 is required for keratinocyte migration on a type Ⅰ collagen matrix[J]. J Cell Biol，1997，137（6）：1445-1457. doi：10.1083/jcb.137.6.1445.
27	BUGGE T H， KOMBRINCK K W， FLICK M J， et al. Loss of fibrinogen rescues mice from the pleiotropic effects of plasminogendeficiency[J]. Cell，1996，87（4）：709-719. doi：10.1016/s0092-8674（00）81390-2.
28	QIANG L， YANG S， CUI Y H， et al. Keratinocyte autophagy enables the activation of keratinocytes and fibroblastsand facilitates wound healing[J]. Autophagy，2021，17（9）：2128-2143. doi：10.1080/15548627.2020.1816342.
29	GOLDMAN R. Growth factors and chronic wound healing： Past， present， and future[J]. Adv Skin Wound Care，2004，17（1）：24-35. doi：10.1097/00129334-200401000-00012.
30	GREENHALGH D G. The role of apoptosis in wound healing[J]. Int J Biochem Cell Biol，1998，30（9）：1019-1030. doi：10.1016/s1357-2725（98）00058-2.
31	TERUEL-MONTOYA R， ROSENDAAL F R， MAR-TÍNEZ C. MicroRNAs in hemostasis[J]. J Thromb Haemost，2015，13（2）：170-181. doi：10.1111/jth.12788.
32	FAHS F， BI X， YU F， et al. New insights into microRNAs in skin wound healing[J]. IUBMB Life，2015，67（12）：889-896. doi：10.1002/iub.1449.
33	MEISGEN F， XU LANDÉN N， WANG A， et al. MiR-146a negatively regulates TLR2-induced inflammatory responses in keratinocytes[J]. J Invest Dermatol，2014，134（7）：1931-1940. doi：10.1038/jid.2014.89.
34	LI D，LI X I， WANG A， et al. MicroRNA-31 promotes skin wound healing by enhancing keratinocyte proliferation and migration[J]. J Invest Dermatol，2015，135（6）：1676-1685. doi：10.1038/jid.2015.48.
35	BISWAS S，ROY S， BANERJEE J， et al. Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds[J]. P Natl Acad Sci USA，2010，107（15）：6976-6981. doi：10.1073/pnas.1001653107.
36	FASANARO P， D’ALESSANDRA Y，DI STEFANO V， et al. MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand Ephrin-A3[J]. J Biol Chem，2008，283（23）：15878-15883. doi：10.1074/jbc.M800731200.
37	CIECHOMSKA M， O’REILLY S， SUWARA M， et al. MiR-29a reduces TIMP-1 production by dermal fibroblasts via targeting TGF-β activated kinase 1 binding protein 1， implications for systemic sclerosis[J]. PLoS One，2014，9（12）：e115596. doi：10.1371/journal.pone.0115596.
38	LI C， ZHU H Y， BAI W D， et al. miR-96 promotes collagen deposition in keloids by targeting Smad7[J]. Exp Ther Med，2019，17（1）：773-781. doi：10.3892/etm.2018.7008.
39	ROOIJ E VAN， SUTHERLAND L B， QI X， et al. Control of stress-dependent cardiac growth and gene expression by a microRNA[J]. Science，2007，316（5824）：575-579. doi：10.1126/science.1139089.
40	GATFIELD D， LE MARTELOT G， VEJNAR C E， et al. Integration of microRNA miR-122 in hepatic circadian gene expression[J]. Gene Dev，2009，23（11）：1313-1326. doi：10.1101/gad.1781009.
41	BALZANO F， DEIANA M， GIUDICI S DEI， et al. miRNA stability in frozen plasma samples[J]. Molecules，2015，20（10）：19030-19040. doi：10.3390/molecules201019030.
42	MALL C， ROCKE D M， DURBIN-JOHNSON B， et al. Stability of miRNA in human urine supports its biomarker potential[J]. Biomark Med，2013，7（4）：623-631. doi：10.2217/bmm.13.44.
43	KELLER A， KREIS S， LEIDINGER P， et al. miRNAs in ancient tissue specimens of the tyrolean iceman[J]. Mol Biol Evol，2017，34（4）：793-801. doi：10.1093/molbev/msw291.
44	IBANEZ-VENTOSO C， VORA M， DRISCOLL M. Sequence relationships among C. elegans，D. melanogaster and human microRNAs highlight the extensive conservation of microRNAs in biology[J]. PLoS One，2008，3（7）：e2818. doi：10.1371/journal.pone.0002818.
45	YANG X， WANG J， GUO S， et al. miR-21 promotes keratinocyte migration and re-epithelialization during wound healing[J]. Int J Biol Sci，2011，7（5）：685-690. doi：10.7150/ijbs.7.685.
46	HU Y，RAO S， WANG Z， et al. Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function[J]. Thera-nostics，2018，8（1）：169-184. doi：10.7150/thno.21234.
47	JOHNSON S M， GROSSHANS H， SHINGARA J， et al. RAS is regulated by the let-7 microRNA family[J]. Cell，2005，120（5）：635-647. doi：10.1016/j.cell.2005.01.014.
48	ESQUELA-KERSCHER A， TRANG P， WIGGINS J F， et al. The let-7 microRNA reduces tumor growth in mouse models of lung cancer[J]. Cell Cycle，2008，7（6）：759-764. doi：10.4161/cc.7.6.5834.
49	NELSON P T， BALDWIN D A， KLOOSTERMAN W P， et al. RAKE and LNA-ISH reveal micro-RNA expression and localization in archival human brain[J]. RNA，2006，12（2）：187-191. doi：10.1261/rna.2258506.
50	XI Y， NAKAJIMA G， GAVIN E， et al. Syste-matic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples[J]. RNA，2007，13（10）：1668-1674. doi：10.1261/rna.642907.
51	MA J， PAN H， ZENG Y， et al. Exploration of the R code-based mathematical model for PMI estimation using profiling of RNA degradation in rat brain tissue at different temperatures[J]. Forensic Sci Med Pathol，2015，11（4）：530-537. doi：10.1007/s12024-015-9703-7.
52	BARTEL D P. MicroRNAs： Target recognition and regulatory functions[J]. Cell，2009，136（2）：215-233. doi：10.1016/j.cell.2009.01.002.
53	BARTEL D P， CHEN C. Micromanagers of gene expression： The potentially widespread influence of metazoan microRNAs[J]. Nat Rev Genet，2004，5（5）：396-400. doi：10.1038/nrg1328.
54	STARK A， BRENNECKE J， BUSHATI N， et al. Animal microRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution[J]. Cell，2005，123（6）：1133-1146. doi：10.1016/j.cell.2005.11.023.
55	LEUNG A K L， SHARP P A. MicroRNA functions in stress responses[J]. Mol Cell，2010，40（2）：205-215. doi：10.1016/j.molcel.2010.09.027.
56	OLEJNICZAK M， KOTOWSKA-ZIMMER A， KRZY-ZOSIAK W. Stress-induced changes in miRNA biogenesis and functioning[J]. Cell Mol Life Sci，2018，75（2）：177-191. doi：10.1007/s00018-017-2591-0.
57	O’CONNELL R M， RAO D S， CHAUDHURI A A， et al. Physiological and pathological roles for microRNAs in the immune system[J]. Nat Rev Immunol，2010，10（2）：111-122. doi：10.1038/nri2708.
58	CHANG L， LIANG J， XIA X， et al. miRNA-126 enhances viability, colony formation, and migration of keratinocytes HaCaT cells by regulating PI3 K/AKT signaling pathway[J]. Cell Biol Int，2019，43（2）：182-191. doi：10.1002/cbin.11088.
59	LI P，HE Q， LUO C， et al. Differentially expressed miRNAs in acute wound healing of the skin： A pilot study[J]. Medicine，2015，94（7）：e458. doi：10.1097/MD.0000000000000458.
60	WANG T， FENG Y， SUN H， et al. miR-21 regulates skin wound healing by targeting multiple aspects of the healing[J]. Am J Pathol，2012，181（6）：1911-1920.
61	彭涛，贾延劼，文全庆，等. 缺氧缺血性脑损伤新生大鼠脑组织小RNA表达的变化[J].中国当代儿科杂志，2010，12（5）：373-376.
	PENG T， JIA Y J， WEN Q Q， et al. Expression of microRNA in neonatal rats with hypoxic-ischemic brain damage[J]. Zhongguo Dangdai Erke Zazhi，2010，12（5）：373-376.
62	SUN T， CHEN X， LIU Z， et al. Expression profiling of microRNAs in hippocampus of rats fol-lowing traumatic brain injury[J]. J Huazhong Univ Sci Technolog Med Sci，2014，34（4）：548-553. doi：10.1007/s11596-014-1313-1.
63	AUNIN E， BROADLEY D， AHMED M I， et al. Exploring a role for regulatory miRNAs in wound healing during ageing： Involvement of miR-200c in wound repair[J]. Sci Rep，2017，7（1）：3257. doi：10.1038/s41598-017-03331-6.
64	TU C， DU T， SHAO C， et al. Evaluating the potential of housekeeping genes， rRNAs， snRNAs， microRNAs and circRNAs as reference genes for the estimation of PMI[J]. Forensic Sci Med Pathol，2018，14（2）：194-201. doi：10.1007/s12024-018-9973-y.
65	LV Y， MA K， ZHANG H， et al. A time course study demonstrating mRNA，microRNA，18S rRNA，and U6 snRNA changes to estimate PMI in deceased rat’s spleen[J]. J Forensic Sci，2014，59（5）：1286-1294. doi：10.1111/1556-4029.12447.
66	秦娟娟，路志勇，崔雪萍，等. MicroRNAs法医学应用的可能性和局限性[J].中国法医学杂志，2017，32（5）：492-496. doi：10.03618/j.issn.1001-5728.2017.05.013.
	QIN J J， LU Z Y， CUI X P， et al. MicroRNAs application in forensic medicine： Possibilities and limitations[J]. Zhongguo Fayixue Zazhi，2017，32（5）：492-496.
67	ZUBAKOV D， BOERSMA A W M， CHOI Y， et al. MicroRNA markers for forensic body fluid identification obtained from microarray[J]. Int J Legal Med，2010，124（3）：217-226. doi：10.1007/s00414-009-0402-3.
68	WANG Z， ZHANG J， LUO H， et al. Screening and confirmation of microRNA markers for forensic body fluid[J]. Forensic Sci Int Genet，2013，7（1）：116-123. doi：10.1016/j.fsigen.2012.07.006.
69	SAUER E， EXTRA A， CACHÉE P， et al. Identification of organ tissue types and skin from forensic samples by microRNA expression analysis[J]. Forensic Sci Int Genet，2017，28：99-110. doi：10.1016/j.fsigen.2017.02.002.
70	CASSE J， MARTRILLE L， VIGNAUD J， et al. Skin wounds vitality markers in forensic pathology： An updated review[J]. Med Sci Law，2016，56（2）：128-137. doi：10.1177/0025802415590175.

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