Detection of Coxsackie virus B_3 gene in myocardium and spleen samples in myocarditis with sudden death

Abstract

Abstract: Objective To detect the Coxsackie virus B_3(CVB_3) gene in myocardium and spleen tissues in viral myocarditis(VMC) with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene.Methods By in situ RT－ PCR,the detection of CVB_3 gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non－ cardiac death group.Results In VMC group,CVB_3 gene－ positive signals were seen in myocardium sections(3 out of total 8 cases， No.1,4,7 cases)and spleen sections(4 out of total 8 cases,No.2,4,6,7 cases).In non－ cardiac death group,no positive signals were detected in both myocardium and spleen tissues.Conclusion Positive detection of CVB_3 gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC. [

Key words: Coxsackievirus B_3, myocarditis, in situ RT－ PCR

CHEN YU－ CHUAN;CHENG JIAN－ DING;MA HUI－ HUI;ET AL.(.DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 000;.DEPARTMENT OF INFECTIOUS DISEASES;THIRD AFFILIATED HOSPITAL;SUN YAT－ SEN UNIVERSITY OF MEDICAL SC. Detection of Coxsackie virus B_3 gene in myocardium and spleen samples in myocarditis with sudden death[J]. , 2001, 0(2): 74-76.

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Detection of Coxsackie virus B_3 gene in myocardium and spleen samples in myocarditis with sudden death

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