Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application

Abstract

Abstract: Objective The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong . Methods DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358、 vWA、 FGA、 D8S1179、 D21S11、 D18S51、 D5S818、 D13S317、 D7S820、 D16S539、 TH01、 TPOX and CSF1PO.The PCR products were analyzed and genotyped by ABI 377－ 96 Sequencer. Results The combined power of discrimination (DP) was 0.999 999 999 999 993 and the combined paternity of exclusion(PE) was 99.999％ . These 13 loci met the Hardy－ Weinberg expectations. Conclusion The two multiplex amplification systems were very useful in forensic case investigation. [

Key words: multiplexing PCR, STR loci, forensic science

LI YUE;WANG SUI－ BAO;LIU CHAO;ET AL. (GUANGZHOU CRIMINAL SCIENCE＆ TECHNOLOGY INSTITUTE;GUANGZHOU 000) [. Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application[J]. , 2001, 0(2): 82-85.

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Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application

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