Abstract: Objective To establish an effective method for testing DNA from the sperm smear. Methods We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA.The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit. Results Although there were a few sperm cells on the slide,it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction. Conclusion The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.

Key words: sperm smear, DNA extracting, STR typing