Abstract: objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system(LCM) is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell.

Key words: laser capure microdissection(LCM), oral epithelial cells, STR-DNA typing