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28 June 2006, Volume 22 Issue 3 Previous Issue    Next Issue
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Relationship between Expression of Nestin in Experimental Brain Contusion and Injury Time JIA DONG-MEI;HE GUANG-LONG;ZHOU YI-WU;DENG WEI-NIAN;SUN LAI-JING;DAI JIA-PEI;LIU LIANG;XU XIAO-HU(.DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;. DEPARTMEN 2006, 0(3): 161-164+.  Abstract ( 1532 )   Objective To observe the alteration of nestin intervals in the experimental traumatic brain injury and investigate its relation to the injury intervals. Methods The rat brain contusion was conducted by falling impact injury. After various survival interval (0.5,6,12 h and 1,3,7,14,28 d), immunohistochemical SP method was used for observing the expression of nestin in the cortex, hippocampal dentate gyrus and the corpus callosum on injury side. Results Expression of nestin positive cells increased at 0.5 h and reached the maximum level in 7 d after brain contusion, then the expression decreased gradually. The intensity of nestin staining in the the cortex and the hippocampal dentate gyrus decreased to normal on 28 d. As to the corpus callosum of injury side it remained weak on 28 d. Conclusion The changes of nestin immunohistochemical staining can be used as an index for forensic estimation of early injury time. Related Articles | Metrics

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The Role of Diaphgram Fatigue in the Death from Hanging with Bound upper Limbs of Rabbits JIANG FU-XUE;JING HAN-LAN;OU GUI-SHENG;LIAO XIN-BIAO;ZHANG YI-JUN;YU YAN-GENG;CHENG JIAN-DING;LI ZHAO-HUI;GUO XIAO-RAN(.DEPARTMENT OF FORENSIC PATHOLOGY;ZHONGSHAN MEDICAL COLLEGE;ZHONG SHAN UNIVERSITY;GUANGZHOU 00;CHINA;.GUANGD 2006, 0(3): 165-167.  Abstract ( 2295 )   Objective To explore the role of diaphgram fatigue in the death from hanging with bound upper limbs of rabbits. Methods Rabbits were hanged with upper limbs bound, then the data of EMGdi. were gathered Results By analyzing power spectral of EMGdi in experiment, we compare the ratio change of H/L between pre-experiment and post-experiment. There is a significance decrease of the ratio of H/L, so it indicates that diaphgram fatigue does exist. Conclusion Diaphgram fatigue plays an important role in the death from hanging with limbs of rabbits bound. Related Articles | Metrics

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Relationship between the Injury Time and DNA Fragmentation and its Quantity in Rat Brain LI FAN;LIAO ZHI-GANG;MA SHU-LING;WANG YE;GUAN PENG;LIU MIN(. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.DEPARTMENT OF FORENSIC MEDICINE OF HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY;LUO 2006, 0(3): 168-170+.  Abstract ( 1691 )   Objective To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion. Methods The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen′s DNA staining conjoined with image analysis technique were used for exploration. Results With the gradually rising of DNA fragmentation,DNA quantity was declining in the brain tissue after contusion. Conclusion TUNEL and Feulgen′s DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue. Related Articles | Metrics

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Validation Studies of the Test Strip of Colloidal Gold-Labeled Anti-Fibronectin EIIIA Splicing Variant for Estimating the Time of Injury ZHANG JIAN-HUA;LIU NING-GUO;CHEN YI-JIU;HUANG XIAO-HUA(INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2006, 0(3): 171-172+.  Abstract ( 2140 )   Objective To evaluate the validation of the test strip of colloidal gold-labeled anti- Fibronectin EIIIA(Fn-EIIIA) Splicing Variant for estimating the time of injury. Methods After skin tissues with variant injury times were collected from corpses, the test strip were used to detect the expression of Fn-EIIIA and its tested results were valued; The arisen autolysis which may influence the results of test were also observed by rats models. Results In no-injured skins, no Fn-EIIIA were detected by the strip, while in all the injured skins it was detected successfully as soon as injured after 1h and it showed more obvious results with the elongating of injured time; No changes made by autolysis were found in our studied time ranges. Conclusion The test strip which used in this study showed to be a sensitive and steady method for estimating the time of injury in forensic science. Related Articles | Metrics

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Relationship between the Postmortem Interval and Nuclear DNA Changes of Heart Muscular Cells in Mice ZHEN JI-LONG;ZHANG XIAO-DONG;NIU QIN-SHAN(DEPARTMENT OF FORENSIC MEDICINE OF CHINESE CRIMINAL COLLEGE;SHENYANG 00;CHINA) 2006, 0(3): 173-176.  Abstract ( 1770 )   Objective To study the changes of DNA degradation in cells of rats and its relationship with the postmortem interval. Method 8 parameters of cell nuclear(including the tail length, the head radius, the percentage of head DNA,the percentage of tail DNA, the tail moment, the olive moment, the head area and the tail area) were chosen to study their changes in the procedure of DNA degradation in myocardium cells in 111 rats at different postmortem interval from 0 to 72 h by using single-cell gel electrophoresis(SCGE)technology and fluorescent microscope combined with auto-analysis-image system method. Results An evident comet tailing was observed in DNA of myocardium cells after electrophoresis, and their changes in all these 8 parameters of cell nuclear were greatly associated with the extension of postmortem interval,which indicate the degradation rate and degree of DNA in the nuclear has a close relationship with postmortem interval in the periods from 0 to 72 h in rats and significant difference were found with those groups (P<0.001). Conclusion The equations, which were concluded from our study, indicate the close relationship of degradation rate and degree of DNA in the nuclear with postmortem interval from 0 to 72 h, and provide an objective and exact new way to estimate the postmortem interval. Related Articles | Metrics

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Observation the Electroporation of Blood cells in Vitro by Scanning Electron Microscope WANG YE;LIU MIN;PENG XUE-MEI;LI FAN;ZHANG FU;ZHANG KUI;LIAO ZHI-GANG(WEST CHINA PRECLINCAL MEDICINE AND FORENSIC MEDICINE SCHOOL;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2006, 0(3): 177-179.  Abstract ( 2077 )   Objective To observe changes on cell membrane in blood cells after they were been electrified. Methods Blood were electrified for 5,10,20,30 s, 1min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes. Results Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1min increased to 40%. Conclusions Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification. Related Articles | Metrics

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Expression of Substance P in Human Laryngopharynx and Gastrointestine in Sudden Erethistic Death SUN HONG-WEI;GAO CAI-RONG;REN GANG-MU;SUN JUN-HONG;LU JIAN;WANG YING-YUAN(SCHOOL OF FORENSIC MEDICINE;SHANXI MEDICAL UNIVERSITY;TAIYUAN 0000;CHINA) 2006, 0(3): 180-182+.  Abstract ( 1757 )   Objective To study the expression of substance P (SP) in human sudden erethistic death, and to seek objective morphological supports to diagnose sudden erethistic death for forensic medicine. Methods The expression of SP was detected with immunohistochemical technique on 15 human laryngopharynx and gastrointestine of sudden erethistic death, and 20 sudden death of heart attack as control. The images of SP were analyzed by image analyzer, and the positive indexes(PI) were calculated. Results SP expression in the experimental groups was significantly stronger than that in the control one (P<0.001). Conclusion SP expression can offer an objective morphological reference support for forensic diagnosing sudden erethistic death. Related Articles | Metrics

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Dissection of Cavernous Nerves in Rat and Model of Neurological Erectile Dysfunction WANG FEI-XIANG;ZHANG LING-LI;ZHU GUANG-YOU(.DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000; 2006, 0(3): 183-185+.  Abstract ( 1718 )   Objectives To identify rat cavernous nerve and establish a rat model of erectile dysfunction caused by injury of cavernous nerve. Methods Ten rats were undergone dissections. 30 experimental rats were randomized into 2 groups, cavernous nerve were identified by electrical stimulation. One month after surgery, rat models were evaluated by electrical stimulation. Results The anatomic structure of cavernous nerve in rats are highly similar to human beings, the erection can be evoked by stimulating cavernous nerves, and after cavernous nerve injury it can not be evoked(P<0.05). Conclusion Because of the highly similarity of cavernous nerve between rats and human beings, so as the suitable price, rat should be used as the ideal ED experimental animal. The model of ED caused by cavernous nerve injury is reliable. Related Articles | Metrics

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Sequences of Off-ladder Alleles of PowerPlex~(TM)16 Kit in Chinese Han Population LU HUI-LING;TAI YUN-CHUN;LIU CHAO;LI HAN-YAN(. DEPARTMENT OF FORENSIC MEDICINE;SUN YAT-SEN UNIVERSITY;GUANGZHOU;00;CHINA;. INSTITUTE OF FORENSIC MEDICINE;SOUTHERN MEDICAL UNIVERSITY;GUANGZHOU;0;CHINA;. GUANGZHOU INSTITUTE OF C 2006, 0(3): 186-189.  Abstract ( 1722 )   Objective To study the sequences of off-ladder alleles of PowerPlexTM16 kit in Chinese Han population and their nomenclature. Methods 10071 Samples from unrelated individuals in Chinese Han population were screened by using PowerPlexTM16 kit and ABI 377 or 3100. The samples showing off-ladder alleles were re-screened with PAGE and the off-ladder alleles were obtained and sequenced. Results 32 off-ladder alleles were found in 11 STR loci, whose frequencies ranged from 0.05‰ to 4.02‰. These alleles were classified as four types: (1)having complete repeat but its length is out of the ladder; (2)having incomplete repeat; (3)having deletion or insertion of one or two base(s) in flanking sequence; (4) having deletion of some segment. Conclusion Off-ladder alleles have various types. It demonstrates varying repteating number of the core unit, and variation in the flanking sequence or core sequence as well.The nomenclature of International Society for Forensic Haemogenetics cannot define these alleles effectively. Related Articles | Metrics

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Heteroplasmy in Human mtDNA Control Region CAO YANG;WAN LI-HUA;GU LIN-GANG;HUANG YING-XUE;XIU CONG-XIAN;HU SHU-HUI;MI CAN(.DIVISION OF THE CRIMINAL INVESTIGATION DEPARTMENT;JIANGSU PROVINCIAL PUBLIC SECURITY BUREAU;NANGJING 00;CHINA;.CHONGQING UNIVERSITY OF MEDICAL SCIENCES;C 2006, 0(3): 190-192.  Abstract ( 2205 )   Objective To observe the length heteroplasmy and point heteroplasmy in human mtDNA control region. Methods The peripheral blood, buccal cell, and single hair shaft from 50 individuals and 16 family members, related in their maternallineage were analyzed by direct sequencing, and clones from 20 individuals whose mtDNA sequences have a T-C transition at 16189 nt were sequenced. Results No point heteroplasmy were observed in peripheral blood, buccal cell, single hair shaft from the same individual, neither in maternally related individuals. Length heteroplasmy was observed in those individuals with a homopolymeric tract and the different clones from the same individual has different proportions of length variants, but the hair shafts from the same individual were very similar to the measurements made from blood DNA. no length heteroplasmy was observed between different tissues from the same individual. Conclusion mtDNA sequences have a characteristic of high consistency and genetic stability, mtDNA sequencing is a suitable tool for forensic applications such as individual identification . Related Articles | Metrics

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A PCR-RFLP Analysis to HLA-B Gene among Chinese Northern Han Populations ZENG ZHAO-SHU;DING MEI;ZHU YUN-LIANG;LIU ZHI-FANG;SUN ZHI-GANG;HUANG HONG-WU(. DEPARTMENT OF FORENSIC MEDICINE;SCHOOL OF BASIC MEDICINE;ZHENGZHOU UNIVERSITY;ZHENGZHOU 00;CHINA;. DEPARTMENT OF FORENSIC SEROLOGY;SCHOOL OF FORENSIC ME 2006, 0(3): 193-195.  Abstract ( 1764 )   Objective To set up the method for analyzing HLA-B gene polymorphism with PCR-RFLP, and to gain population data among northern Chinese Hans of HLA-B′s restricted fragments after NlaIII digestion, and to achieve application in forensic medicine practice. Methods Sample DNA was extracted by the phenol/chloroform extraction method, 943 bp-long fragments containing HLA-B exon 2 and 3 were got by PCR. The endonuclease NlaIII was applied to cut the PCR products into polymorphic fragments shorter than 943bp, then PAGE and silver staining were used to detect the digestion results, finally the digestion sites were assured by DNA sequencing. Results Along 943bp-long PCR products, 14 length-different fragments, 20 kinds of fragment combinations were got and 6 cutting site were observed after NlaIII digestion. Conclusion HLA-B gene was highly polymorphic among Chinese northern Hans. Even with only one endonuclease, 14 restricted fragments were got and the PIC was great. Such a HLA-B PCR-RFLP analysis will have values in forensic medicine applications. Related Articles | Metrics

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DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection GU LI-HUA;ZHANG CHENG;CHEN LIAN-KANG;ZHEN HUI-FEN;CHENG LI;ZHOU HUAI-GU(SHANGHAI INSTITUTE OF FORENSIC SCIENCE;SHANGHAI;000;CHINA) 2006, 0(3): 196-197+.  Abstract ( 1942 )   objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system(LCM) is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell. Related Articles | Metrics

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Genetics of Heteroplasmy in the mtDNA Control Region among the Chinese Han Population JIANG QIONG-CHENG;TONG DA-YUE;SUN HONG-YU;OU JING-HUA;CHEN LI-XIAN;WU XIN-YAO (DEPARTMENT OF FORENSIC BIOLOGY;FACULTY OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COLLEGE SUN YAT-SEN UNIVERSITY;GUANGZHOU 00;CHINA) 2006, 0(3): 198-203.  Abstract ( 2047 )   Objective To explore the distribution and genetic pattern of heteroplasmy of mtDNA control region among Chinese Han population. Methods The human mtDNA control region was amplified into 6 amplicons overlapped partially each other. Then these amplicons were analyzed by DHPLC which we developed to detect low heteroplasmic signals. Results There were 51 heteroplasmic cases(34%) found from different tissues of 150 unrelated individuals of the Chinese Han population. mtDNA heteroplasmy shows non-uniform distribution in various tissues. The highest occurrence of heteroplasmy was in brain tissues (50/150) and myocardium(48/150), the lowest was in bone tissues (22/150). 36 sites of heteroplasmy were identified in our samples. Three sites of mtDNA heteroplasmy rarely co-existed in one individual. No sex differences were detected in the frequency of mtDNA heteroplasmy. No change in the mtDNA heteroplasmy profile was detected of blood samples from the same individuals within 2 years. Individuals older than 41 years showed a heteroplasmy frequency significantly higher than their younger counterparts. Members from the same maternal pedigree in a family can share the same sites of mtDNA heteroplasmy but may have different heteroplasmy contents at those sites. Conclusion DHPLC is a highly sensitive technique in detecting heteroplasmy. mtDNA heteroplasmy widely exists in the Chinese Han population. The results shown here could potentially have a guidable value in forensic individual identification and parentage testing. Related Articles | Metrics

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Forensic Application of 9 Y-STRs Fluorescence-labeled Multiplex Amplification System SHI MEI-SEN;LI YING-BI;DENG JIAN-QIANG;JI QIANG;YU XIAO-JUN;HOU YI-PING(.DEPARTMENT OF FORENSIC MEDICINE;SHANTOU UNIVERSITY MEDICAL COLLEGE;SHANTOU 0;CHINA;.WEST CHINA PRECLINIAL AND FORENSIC COLLEGE;SICHUAN UNIVERSITY;CHENGDU 00; 2006, 0(3): 204-206+.  Abstract ( 1635 )   Objective In order to increase significantly the discriminatory potential of Y-STR systems available to the forensic community, we have developed a system capable of simultaneously amplifying 9 Y-STR loci by fluorescence-labeled multiplex PCR technique. Methods Primers of STR loci DYS434, GATA-A10, DYS438 and DYS439 were labeled with 6-FAM, primers of STR loci DYS531, DYS557, DYS448 were labeled with HEX, and primers of STR loci DYS456, DYS444 were labeled with TAMRA, respectively. PCR products were analyzed using capillary electrophoresis and GeneScan Software on the ABI Prism310 DNA Analyzer. Series experiments were carried out to evaluate the useful value in forensic application such as the sensitivity, male specificity and genotyping DNA different tissues of the same individual. Results 9 Y-STR loci were exactly determined following optimization of the polymerase chain reaction. In a sample of 120 males, a total of 105 different haplotypes was identified, 97 of them being unique. Overall, haplotype diversity was 0.996 8. It was proved that genotyping of these 9 Y-STR loci in some sexual crime should be prior to that of automosomal STR. Conclusion The results suggest that the newly constructed 9-plex will be very powerful for establishing Y-STR database, population genetic studies and mixture stains identification. Related Articles | Metrics

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Haplotypes of DXS10011 and DXS8377 in Guangdong Han Individuals LU DE-JIAN;CHEN SHU-CHUN;WANG XUE-WEN;LIU QIU-LING;LU HUI-LING(DEPARTMENT OF FORENSIC SEROLOGY;ZHONGSHAN-MEDICAL COLLEGEL OF SUN YAT-SEN UNIVERSITY;GUANGZHOU 00;CHINA) 2006, 0(3): 207-209.  Abstract ( 1939 )   Objective To investigate genetic polymorphism of two X chromosome specific STR: DXS10011 and DXS8377 in male samples from Guangdong Han population. Methods The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining. Results Among 113 samples, 20 alleles were found for DXS1011 and 12 alleles for DXS8377. Also, 72 DXS10011-DXS8377 haplotypes were shown. The most common haplotypes only occurred three times. When only female children were tested in motherless case, the exclusion probability of paternity was 0.9588 for DXS10011-DXS8377 haplotypes. Investigations in 83 family trios with female children and 29 pedigrees with two children suggested a co-dominant X-linked inheritance; mutations were not found. Conclusion Our data indicate that DXS10011 and DXS8377 are highly informative X chromosome markers for complicated kinship analysis. Related Articles | Metrics

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Polymorphisms of Eleven Y-chromosome STR loci and Forensic Application in Yunnan Han Population ZHANG XIAO-HONG;WU WEI-WEI;TANG JIAN-XIN;QIAN GUANG-LI;ZHANG XIAO-MING(.PANYU DIVISION OF GUANGZHOU PUBLIC SECURITY BUREAU;GUANGZHOU 00;CHINA;.DEPARTMENT OF CRIMINAL INVESTIGATION;ZHEJIANG PROVINCIAL PUBLIC SECURITY BUREAU;HANGZHOU 000;CHIN 2006, 0(3): 210-212+.  Abstract ( 1877 )   Objective To abtain the genetic polymorphisms data of eleven Y-chromosome STR loci in Yunnan Han population, and to evaluate its forensic application values, which will provide the base data for forensic application. Methods DYS391,DYS389Ⅰ,DYS439,DYS389Ⅱ,DYS438,DYS437,DYS19,DYS392,DYS393,DYS390 and DYS385a/b loci were amplified by using Powerplex?誖Y PCR amplification Kit and were analyzed by 310 genetic analyzer. 201 unrelated male individuals of Han population in Yunnan were investigated to determine the distributions of allele frequencies and haplotypes. Results 3,5,6,8,5,4,5,8,4,7 alleles were found across the Y-STR loci except DYS38556 haplotypes were found across DYS385a/b locus. The minimum GD value was 0.4273(DYS438),and the maximum was 0.9747(DYS385a/b). 175 haplotypes were found, of which 154 were unique, 16 were found in 2 individuals,5 were found in 3 individuals, and the observed haplotypes diversity value was 0.9984. Conclusion The 11 Y-STR loci are highly polymorphic and are suitable for forensic personal identification and paternity testing in Yunnan Han population . Related Articles | Metrics

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Homologous Amelogenin Gene Test of Archaeological Samples ZHANG HU-QIN;YANG ZHOU-QI;LIU FANG-E;ZHANG JIN;ZHAO WEN-MING(.THE KEY LABORATORY OF BIOMEDICAL INFORMATION ENGINEERING OF MINISTRY OF EDUCATION;SCHOOL OF LIFE SCIENCE AND TECHNOLOGY;XI′AN JIAOTONG UNIVERSITY;XI′AN 00;CHINA;.THE CENTER 2006, 0(3): 213-216.  Abstract ( 1763 )   Objective Based on the sequence differences of Amelogenin homologous gene in the X and Y chromosomes, a pair of specific primers was designed to identify the sex of archaeological samples. Methods Ancient DNA fragments were extracted from the bones and teeth of sacrificial slaves with an improved method that combines phenol-chloroform extraction, silicon dioxide adsorption with ultrafiltration concentration. The polyacrylamide gel electrophoresis (PAGE) was used to detect PCR products. Results Seven in sixteen samples from eight graves showed positive results and the targeted segments were visible: a male with two bands of 106 bp (Amel-X) and 112 bp (Amel-Y), while a female with only one band of 106 bp (Amel-X). Ancient DNA analyzing results from tooth samples are more marked than that from bones. Conclusion The improved extraction method is more effective for ancient DNA extraction, which reduced the PCR inhibitors and lowered experimental costs. The sex determination technology based on Amelogenin homologous gene is an important and feasible method in the molecular archaeological research. Related Articles | Metrics

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Dynamic Determination of the Medicine Concentration of Poisoned Blood of Acephate CHU JIAN-XIN;SHEN JU-HUA;JIANG WEN-HUI(.THE PEOPLE′S PROCURATORATE OF ZHEJIANG PROVINCE;HANGZHOU 00;CHINA;.THE PEOPLE′S HOSPITAL OF DEQING;DEQING;CHINA;.THE PEOPLE′S PROCURATORATE OF HANGZHOU;HANGZHOU 00;CHINA) 2006, 0(3): 217-219.  Abstract ( 2155 )   Objective To observe rule of medicine concentration of blood and the last concentration that through hemoperfusion after poisoned by acephate. Methods Utilizeng the patient annual bonus venous blood in hospital emergency room, the content of acephate in plasma was analyzed by gas chromatography. Results After hemoperfusion, the concentration of acephate showed a rapid drop and the characteristic that the concentration drops quicker if medicine concentration of blood before hemoperfusion is higher. Conclusion Hemoperfusion is able to rapidly reduce the concentration of acephate in blood, its speed is determined by initial concentration and the beginning time of hemoperfusion etc. Related Articles | Metrics

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Retrospective Analysis of Unnatural Death in One District of Shanghai City from the Years 1995 to 2004 JI JIE-MING;WANG YI (.LUWAN BRANCH OF BUREAU OF PUBLIC SECURITY OF SHANGHAI MUNICIPALITY;SHANGHAI 000;CHINA;.LUWAN CENTER FOR DISEASES CONTROLLING AND PREVENTION;SHANGHAI 000;CHINA) 2006, 0(3): 220-223.  Abstract ( 2039 )   According to the criterion of International Statistical Classification of Diseases and Related Health Problems Revisions, From 1995 to 2004, Data of unnatural death of residents were analyzed. The results showed that the annual rate of unnatural death was from 4.9 ～4.5 , and females were of higher rate than males(1.03 to 1.36 times). The three leading causes of unnatural death were falling,traffic accident and suicide. Our date indicate that, unnatural death could influence people′s health and life quality,much attentions should be put into its controlling, prevention and other related research. Related Articles | Metrics

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The Advances in the Neuropathology of Sudden Infant Death Syndrome JIN BO;HUANG RUI-RUN;BAI PENG;LIAO ZHI-GANG(.WEST CHINA PRECLINIAL AND FORENSIC COLLEGE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.BUREAU OF PUBLIC SECURITY OF RUIAN;WENZHOU 00;CHINA) 2006, 0(3): 230-232.  Abstract ( 1898 )   Sudden infant death syndrome is known as sudden death in 12 months postnatal without obvious cause,the cause of death and differential diagnosis of still couldn′t be indefinite so far.In this paper,the recent studies on the SIDS neuropathology using immunohisto- chemical technology were reviewed in order to suggest the lethal patho-physiologic derangement or mechanism in SIDS involves dysfunction of sleep-related cardio-respiratory homeostatic controls or failure to arouse. Thus the main cause of SIDS lies in central nerve system,but not unique cause. Related Articles | Metrics