Relationship between the Injury Time and DNA Fragmentation and its Quantity in Rat Brain

Abstract

Abstract: Objective To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion. Methods The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen′s DNA staining conjoined with image analysis technique were used for exploration. Results With the gradually rising of DNA fragmentation,DNA quantity was declining in the brain tissue after contusion. Conclusion TUNEL and Feulgen′s DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.

Key words: brain contusion, TUNEL staining, Feulgen′s DNA staining, timing of injury

LI FAN;LIAO ZHI-GANG;MA SHU-LING;WANG YE;GUAN PENG;LIU MIN(. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.DEPARTMENT OF FORENSIC MEDICINE OF HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY;LUO. Relationship between the Injury Time and DNA Fragmentation and its Quantity in Rat Brain[J]. , 2006, 0(3): 168-170+.

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Relationship between the Injury Time and DNA Fragmentation and its Quantity in Rat Brain

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