Abstract: Objective To evaluate the validation of the test strip of colloidal gold-labeled anti- Fibronectin EIIIA(Fn-EIIIA) Splicing Variant for estimating the time of injury. Methods After skin tissues with variant injury times were collected from corpses, the test strip were used to detect the expression of Fn-EIIIA and its tested results were valued; The arisen autolysis which may influence the results of test were also observed by rats models. Results In no-injured skins, no Fn-EIIIA were detected by the strip, while in all the injured skins it was detected successfully as soon as injured after 1h and it showed more obvious results with the elongating of injured time; No changes made by autolysis were found in our studied time ranges. Conclusion The test strip which used in this study showed to be a sensitive and steady method for estimating the time of injury in forensic science.

Key words: time of injury, fibronectin EIIIA, autolysis, colloid gold, forensic medicine