Expression of CGRP in Rat's Skin and Post-traumatic Interval

Abstract

Abstract: Objective To study the expression of calcitonin gene-related peptide (CGRP) in rat's skin after injury. Methods Immunohistochemistry technique, auto-image analysis, and in situ hybridization were used to study the expression of CGRP protein and mRNA at different post-traumatic time intervals in both antemortem and postmortem groups. Results The expression of CGRP in neurofibrils was higher than that in the control (P<0.05). However, in postmortem injury group, there were no significant changes in anti-CGRP immounreactivities in neurofibrils compared to the control group (P>0.05). Further, the expression of CGRP could be detected increasing in 1h, and reached maximum in 4 h, then returned to normal. Conclusion The expression of CGRP in rat's skin is time dependent after skin injury. It could used as a bio-marker to determine the time of injury.

Key words: time of injury, calcitonin gene-related peptide (CGRP), immunohistochemistry, in situ hybridization

ZHOU JIAN-QING;WANG YONG-ZHONG;JIANG HONG-YAN;KONG JIE(.THE CRIMINAL TECHNIQUE INSTITUTE OF ZHENJIANG PUBLIC SECURITY BUREAU;ZHENJIANG 00;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF JIANGSU UNIVERSITY;ZHENJIANG 00;CHINA;. Expression of CGRP in Rat's Skin and Post-traumatic Interval[J]. , 2007, 0(3): 193-195+.

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[2]	WU Mao-wang, SU Li, HE Fang, et al. Differential Expression of Integrin β1 in Two Brain Injury Models of Rats [J]. 法医学杂志, 2020, 36(4): 502-506.
[3]	CHEN Jing-wei, WANG Peng-fei, ZHANG Meng-zhou1，et al.. Relationship between Expression Changes of CB2R and Wound Age of Brain Contusion in Mice [J]. 法医学杂志, 2019, 35(2): 136-142.
[4]	ZHAO Jian-xin, JIN Xin, HUANG Jun-jie，et al.. Time-dependent Expression and Distribution of AChE during the Skin Incised Wound Healing in Mice [J]. 法医学杂志, 2019, 35(2): 143-148.
[5]	WEI SHU-RONG, CHEN XIN-SHAN, CHEN HUANG-FENG, ET AL.. Expression of Fas Protein of Myocardium in Dilated Cardiomyopathy [J]. , 2012, 28(4): 252-255.
[6]	YUAN XIANG-TIAN, PENG XUE, JIN ZHAN-FENG. Expression of FAPα and TGF-β1 in Sudden Deaths due to Acute Myocardial Ischemia [J]. , 2012, 28(1): 18-20.
[7]	WANG FU-YUAN, LI RU-BO, LIANG HONG-XIA, ET AL. Homer Protein Expression after Focal Brain Contusion in Rats [J]. , 2011, 27(6): 409-412.
[8]	PENG XUE, JIN ZHAN-FENG, SHAO HONG-JIANG, ET AL. Immunohistochemical Expression of HO-1 in Traumatic Human Brain Tissue [J]. , 2010, 26(5): 325-327,331.
[9]	ZHU JIAN-HUA, HU YONG-LIANG, ZHANG WU, ET AL. Effects of Triptolide on Hypothalamic-pituitary-adrenal Axis of Rats [J]. , 2010, 26(4): 260-265.
[10]	PEI MING;SUN JUN-HONG;GAO CAI-RONG(.SCHOOL OF FORENSIC MEDICINE;SHANXI MEDICAL UNIVERSITY;TAIYUAN 0000;CHINA;.QUANSHAN BRANCH OF XUZHOU PUBLIC SECURITY BUREAU;XUZHOU 00;CHINA). Changes of ICAM-1 mRNA Expression in Rats' Skeletal Muscle after Contusion [J]. , 2009, 0(3): 161-163.
[11]	GUO XIAO-CHONG;LI RU-BO;LIANG HONG-XIA;WANG FU-YUAN;WU XU (. DEPARTMENT OF FORENSIC PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA;. KEY LAB OF LABORATORY TRANSGENIC ANIMAL OF LIAONING PROVINCE;DEPA. Expression of Matrix Metalloproteinase-3 after Brain Contusion in Rats [J]. , 2009, 0(1): 1-5.
[12]	CHEN ZONG-YUN;WANG JING-YU;LI YONG-HONG (DEPARTMENT OF FORENSIC MEDICINE;WANNAN MEDICAL COLLEGE;WUHU 000;CHINA). The Expression of NGF after Rat Diffuse Axonal Injury [J]. , 2008, 0(3): 172-174.
[13]	YANG DA-LI;GUAN DA-WEI;ZHANG JIAN-JUN(SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA). Expressions and Time-dependent Changes of FAK and phospho-FAK during Skin Incised Wound Healing in Mouse [J]. , 2008, 0(2): 81-84.
[14]	XIONG XIAO-MING;DENG SHI-XIONG(.DEPARTMENT OF PATHOLOGY;LUZHOU MEDICAL COLLEGE;LUZHOU 000;CHINA;.DEPARTMENT OF FORENSIC PATHOLOGY;CHONGQING MEDICAL UNIVERSITY;CHONGQING 000 CHINA). Expression of cTnT in Rabbit Hearts during Myocardial Ischemia and its PostMortem Stability [J]. , 2008, 0(2): 90-93+1.
[15]	YAO YA-NAN;LU HUI-LING;CHEN SEN;ZHENG JING;ZHANG YA-QING (.DEPARTMENT OF FORENSIC BIOLOGY;ZHONGSHAN SCHOOL OF MEDICINE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA;.THE FIRST AFFILIATED HOSPITAL OF SUN YAT-SEN UNIVERSITY;GUANGZHOU;000;. Detection of MMP-11 from Menstrual Blood Using Immunohistochemistry [J]. , 2008, 0(1): 32-33+8.

Expression of CGRP in Rat's Skin and Post-traumatic Interval

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