Analysis of Appropriate Amount of Template DNA for Sinofiler Kit by Real Time Quantitative PCR Technique

Abstract

Abstract: Objective To explore the appropriate amount of template DNA for Sinofiler Kit.Methods The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.Results It was shown that 1.29-1.51 ng of template DNA in 12.5 μL reaction volume was optimal for STR genotyping with Sinofiler Kit.Conclusion Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.

Key words: forensic biology, fluorescent quantitative PCR, quantification, Sinofiler kit

LI CHENG-TAO;GUO HONG;LIN YUAN;LIU YAN;QUE TING-ZHI;LI LI(.SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE;INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;.SCHOOL OF LIFE SCIENCES;FUDAN UNIVERSITY;SHANGHAI 00;C. Analysis of Appropriate Amount of Template DNA for Sinofiler Kit by Real Time Quantitative PCR Technique[J]. , 2008, 0(2): 129-130+.

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Analysis of Appropriate Amount of Template DNA for Sinofiler Kit by Real Time Quantitative PCR Technique

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