DNA Polymorphism Detection of Cannabis Using Amplified Fragment Length Polymorphism

›› 2008, Vol. 0 ›› Issue (5): 330-332.

DNA Polymorphism Detection of Cannabis Using Amplified Fragment Length Polymorphism

GUO JIA1,PEI LI2,PENG JIAN-XIONG2,ZHAI HONG3,ZHANG GUI-QIN1,JIE QIN3,TU ZHENG2 (1. CHINESE PEOPLE′S PUBLIC SECURITY UNIVERSITY,BEIJING 100038,CHINA; 2. INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF PUBLIC SECURITY,P.R.C,BEIJING 100038,CHINA; 3. CHINA AGRICULT

Online:2008-10-25 Published:2008-10-28

Abstract

Abstract: Objective To screen the AFLP primers with good diversity to distinguish various species of Cannabis. Methods The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations. Results A total of 285 AFLP bands were obtained using five primer combinations with better diversity,among which 99 bands were polymorphic and 10 bands were special,with 47-76 bands amplified in each pair of primers. Conclusion AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.

Key words: forensic biology, genetic diversity, Cannabis, AFLP

GUO JIA;PEI LI;PENG JIAN-XIONG;ZHAI HONG;ZHANG GUI-QIN;JIE QIN;TU ZHENG (. CHINESE PEOPLE′S PUBLIC SECURITY UNIVERSITY;BEIJING 000;CHINA;. INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF PUBLIC SECURITY;P.R.C;BEIJING 000;CHINA;. CHINA AGRICULT. DNA Polymorphism Detection of Cannabis Using Amplified Fragment Length Polymorphism[J]. , 2008, 0(5): 330-332.

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DNA Polymorphism Detection of Cannabis Using Amplified Fragment Length Polymorphism

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