Abstract: Objective To investigate the diagnostic significance of basophil activation test （BAT） in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 （MC-CPA3）, and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to ?茁-lactam drugs and non-ionic contrast media. Methods The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA. Results The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and ?茁-lactam drugs were significantly higher than that in normal immune group （P<0.05）. Conclusion There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.

Key words:  forensic pathology, anaphylaxis, non-ionic contrast media, basophil activation test, CD63, mast cell carboxypeptidase A3, SC5b-9