Archive

28 June 2015, Volume 31 Issue 3 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Relation between Injury Time and the Expression of COX6C mRNA in Skeletal Muscle of Rats after Contusion SUN JUN-HONG, ZHANG LEI, WANG XIAO-WEI, ET AL. 2015, 31(3): 177-180.  DOI: 10.3969/j.issn.1004-5619.2015.03.001 Abstract ( 886 )   Objective To investigate the relation between injury time and the expression of cytochrome c oxidase subunit Ⅵc （COX6C） mRNA in skeletal muscle of rat after contusion. Methods A total of fifty-four SD rats were divided into the control group and the contusion groups （0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion）, randomly. The contusion model was established by free fall drop of gravity hammer. At corresponding time point after contusion, the regular histology was examined and expression level of COX6C mRNA was tested by real-time PCR after extraction of total RNA from the tissues. Results The main pathological features of 6 h after injury included edema and hemorrhage in myocytes with no inflammatory cells found. After 6 hours, the findings included myocyte degeneration and necrosis, inflammatory cells infiltration, and fibrous connective tissue proliferation in the contused zone. The expression level of COX6C mRNA was higher than that of the control group within 6 h after contusion. The expression level was lower than that of the control group from 6-36 h after contusion. Conclusion The level of COX6C mRNA expresses in a regular way after contusion. It may be useful for estimating wound age in combination with the results of pathological features. Related Articles | Metrics

Select

Changes of Serum IgE and Tryptase in Anaphylactic Shock Rats MI LI, GAO WEI-MIN, DU ZHONG-BO, ET AL. 2015, 31(3): 181-184.  DOI: 10.3969/j.issn.1004-5619.2015.03.002 Abstract ( 873 )   Objective To explore the changes of serum IgE and tryptase caused by anaphylactic shock rats and discuss the relation to PMI and preservative environment of corpse and specimen. Methods Rats were used for establishing anaphylactic shock models and randomly divided into room temperature group, refrigeration group, frozen group, manual hemolysis group, specimen preservation group. And the control group was also established. The blood samples were collected after rats were sacrificed. The degree of hemolysis was graded according to the color of the upper layer of the serum. The mass concentration of IgE and tryptase in each group was detected by ELISA. Results The levels of serum IgE and tryptase in anaphylactic shock dead rats were higher than that of the control group. Room temperature and frozen made obviously differences on the levels of serum IgE and tryptase with various PMI. The levels of serum IgE and tryptase in refrigeration group showed relatively stable. The levels of serum tryptase and IgE were elevated with differently increasing hemolysis. The levels of serum IgE and tryptase showed no obvious changes during the specimen kept under different temperature conditions for 25 days. Conclusion Serum IgE and tryptase obviously increased in anaphylactic shock rats. However, the levels were influenced by PMI and environmental temperature, especially under the conditions of room temperature and frozen. Related Articles | Metrics

Select

Effect of Heroin on DLG4 Expression in Hippocampus, Amygdala and Frontal Cortex of Rats LUO LIANG-MING, GONG , QUN , LIU JIAN-FENG, ET AL. 2015, 31(3): 185-187.  DOI: 10.3969/j.issn.1004-5619.2015.03.003 Abstract ( 900 )   Objective To observe the expression of discs large homolog 4 （DLG4） protein in hippocampus, amygdala and frontal cortex of rats and evaluate postsynaptic density in heroin dependence. Methods The rat heroin dependent model was established by increasing intraperitoneal injection of heroin. DLG4 proteins in hippocampus, amygdala and frontal cortex of heroin dependent 9, 18, 36 days rats were detected with immunohistochemical staining and compared with that in the control group. Results DLG4 proteins in hippocampus, amygdala and frontal cortex were gradually reduced with extension of heroin dependent time. Conclusion Heroin dependence can affect postsynaptic density of hippocampus, amygdala and frontal cortex. The changes become more apparent with extension of heroin dependence time. Related Articles | Metrics

Select

Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media ZHANG HAO-YUE, XU SU-JUN, TANG XIAO-XIAN, DENG 2015, 31(3): 188-190.  DOI: 10.3969/j.issn.1004-5619.2015.03.004 Abstract ( 826 )   Objective To investigate the diagnostic significance of basophil activation test （BAT） in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 （MC-CPA3）, and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to ?茁-lactam drugs and non-ionic contrast media. Methods The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA. Results The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and ?茁-lactam drugs were significantly higher than that in normal immune group （P<0.05）. Conclusion There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media. Related Articles | Metrics

Select

Postmortem MSCT Analysis and Identification of Fall from Height: One Case Report HAN SHUN-QI, WAN LEI, HUANG PING, ET AL. 2015, 31(3): 191-195.  DOI: 10.3969/j.issn.1004-5619.2015.03.005 Abstract ( 999 )   Objective To explore the application value of postmortem multi-slice spiral computed tomography （MSCT） in cases of fall from height through observing and analyzing the injury features of the fall and reconstructing the process of the fall based on the the above procedure. Methods One real fatal case due to fall from height was fully examined using MSCT and three-dimensional reconstruction technique. Analyzing the manner and cause of death through combination of MSCT and systemic autopsy was also implemented. The differences between autopsy and MSCT in getting information of injuries were compared. Results Fractures involving multiple body regions and liver rupture were found through MSCT and three-dimensional reconstruction. The autopsy got the same results with imageological examination. The case of death was deduced to be fall leading to systemic polytrauma. Conclusion Application of MSCT can be used as the complimentary for traditional autopsy in the analysis of injury manner of fall from height. Related Articles | Metrics

Select

Relation between Body Height and Combined Length of Manubrium and Mesosternum of Sternum Measured by CT-VRT in Southwest Han Population LUO YING-ZHEN, TU MENG, FAN FEI, ET AL. 2015, 31(3): 196-199.  DOI: 10.3969/j.issn.1004-5619.2015.03.006 Abstract ( 1024 )   Objective To establish the linear regression equation between body height and combined length of manubrium and mesosternum of sternum measured by CT volume rendering technique （CT-VRT） in southwest Han population. Methods One hundred and sixty subjects, including 80 males and 80 females were selected from southwest Han population for routine CT-VRT （reconstruction thickness 1 mm） examination. The lengths of both manubrium and mesosternum were recorded, and the combined length of manubrium and mesosternum was equal to the algebraic sum of them. The sex-specific linear regression equations between the combined length of manubrium and mesosternum and the real body height of each subject were deduced. Results The sex-specific simple linear regression equations between the combined length of manubrium and mesosternum （x3） and body height （y） were established （male： y=135.000+2.118 x3 and female： y=120.790+2.808 x3）. Both equations showed statistical significance （P<0.05） with a 100% predictive accuracy. Conclusion CT-VRT is an effective method for measurement of the index of sternum. The combined length of manubrium and mesosternum from CT-VRT can be used for body height estimation in southwest Han population. Related Articles | Metrics

Select

Analysis of GHB and Its Precursors in Urine and Their Forensic Application SHI YAN, CUI XIAO-PEI, XIANG PING, ET AL. 2015, 31(3): 200-203.  DOI: 10.3969/j.issn.1004-5619.2015.03.007 Abstract ( 1093 )   Objective To establish the method to analyze γ-hydroxybutyric acid （GHB） and its precursors 1,4-butanediol （1,4-BD） and gamma-butyrolactone （GBL） in urine through LC-MS/MS and provide evidence for related cases. Methods GHB-d6 and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring （MRM） mode. Results The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix effects were higher than 80%. Conclusion The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB. Related Articles | Metrics

Select

Forensic Analysis of Melamine-tainted Milk Powder Poisoning: Six Fatal Cases ZHAO JIE, LI HONG-TAO 2015, 31(3): 204-205.  DOI: 10.3969/j.issn.1004-5619.2015.03.008 Abstract ( 972 )   Objective To analyze the medical records of poisoned children to provide references for the forensic identification of melamine-tainted milk powder poisoning. Methods Medical records of six fatal cases of consuming some brand melamine-tainted milk powder were studied, specifically the poisoning symptoms, medical imaging, blood biochemical tests, treatment and prognosis. Results The major medical problems of these eight-month sick infants were urinary tract obstruction caused by urinary tract calculi. The poisoned infants developed oliguria, anuria and other symptoms, eventually, acute renal failure or other complications leaded to death. The serum BUN and Cr abnormally increased. Conclusion By considering the toxicological effects of melamine, it was concluded that the deaths of these sick infants were related to the melamine poisoning. Related Articles | Metrics

Select

Analysis of 59 Anaphylactic Death Cases LI ZHENG-DONG, LIU NING-GUO, ZHAO ZI-QIN, ET AL. 2015, 31(3): 206-210.  DOI: 10.3969/j.issn.1004-5619.2015.03.009 Abstract ( 1021 )   Objective To analyze the cases of anaphylactic death cases and explore the standards of judicial expertise of anaphylactic death for providing evidence for judicial expertise. Methods Fifty-nine cases death due to allergic reaction in Shanghai were collected. And details of medical history, clinical manifestation of anaphylactic reaction and postmortem examination findings were reviewed for all cases. Results In the 59 cases, there were 58 cases died from drug allergy, including 77.6% of them were antibiotics. The rates of treating in standard hospital and illegal clinic were 37.3% and 61.0%, respectively. The allergic symptoms were dyspnea and facial cyanosis. The time from contacting allergens to death ranged from 1 min to 3 d. The concentration of total serum IgE ranged from 50 to 576.92 IU/mL. The results of clinical manifestation and pathological anatomy had obviously changes. Conclusion Based on the exclusion of all other cause of death and synthetically analysis of details of cases, medical history, clinical manifestation and anatomy, the conclusion of anaphylactic death can reached. The details of cases including clinical history, exposure to allergens, and clinical manifestation play an important role in diagnosis of anaphylactic death. Related Articles | Metrics

Select

Genetic Polymorphisms of 21 Autosomal STR Loci of Fujian Han Population LIAN HUI-HUI, GE WEN-DONG, LIN FENG, ET AL. 2015, 31(3): 211-214.  DOI: 10.3969/j.issn.1004-5619.2015.03.010 Abstract ( 1083 )   Objective To investigate the genetic polymorphisms of 21 autosomal STR loci of Fujian Han population and evaluate the forensic application value of GlobalFilerTM Express kit. Methods Amplified with GlobalFilerTM Express kit, DNA samples were obtained from 741 unrelated individuals of Fujian Han population. The population genetics parameters of 21 autosomal STR loci were calculated. Results The 21 autosomal STR loci were found to be no deviation from Hardy-Weinberg equilibration （P>0.05） and relatively abundant in high polymorphism. Heterozygosity ranged from 0.589 to 0.914, power of discrimination ranged from 0.754 to 0.992, polymorphic information content ranged from 0.520 to 0.940, and power of exclusion ranged from 0.278 to 0.825. The SE33 locus was the highest degree in polymorphism. Conclusion The 21 STR loci of GlobalFilerTM Express kit have high value in discrimination power and can be useful in personal identification and paternity test in Fujian Han population. Related Articles | Metrics

Select

Research Progress of Event-related Potential in Mild Cognitive Impairment JI MENG-MENG, MENG HUAN-HUAN, LUO BIN, ET AL. 2015, 31(3): 215-218.  DOI: 10.3969/j.issn.1004-5619.2015.03.011 Abstract ( 827 )   Mild cognitive impairment caused by craniocerebral trauma is the key points and difficulties in judicial authentication. This article has comparative analysis of each mode of event-related potential （classical Oddball, Eriksen flanker task and so on）, which can provide a more objective method for such craniocerebral trauma cases in clinical forensic judicial authentication. Related Articles | Metrics

Select

Development and Application of Metabonomics in Forensic Toxicology YAN , HUI , SHEN , MIN 2015, 31(3): 219-226.  DOI: 10.3969/j.issn.1004-5619.2015.03.012 Abstract ( 992 )   Metabonomics is an important branch of system biology following the development of genomics, transcriptomics and proteomics. It can perform high-throughput detection and data processing with multiple parameters, potentially enabling the identification and quantification of all small metabolites in a biological system. It can be used to provide comprehensive information on the toxicity effects, toxicological mechanisms and biomarkers, sensitively finding the unusual metabolic changes caused by poison. This article mainly reviews application of metabonomics in toxicological studies of abused drugs, pesticides, poisonous plants and poisonous animals, and also illustrates the new direction of forensic toxicology research. Related Articles | Metrics

Select

Research Progress on Forensic Entomotoxicology LIU ZHI-JIANG, DI XIAN-DUN, GUAN LING, ET AL. 2015, 31(3): 227-229.  DOI: 10.3969/j.issn.1004-5619.2015.03.013 Abstract ( 1057 )   Forensic entomotoxicology is a branch of forensic medicine, which applies entomology, toxicology and other related studies to solve the poisoning cases. It has an obvious advantage in the investigation on poisoning death. Based on the expounding definition and research of entomotoxicology, this paper reviews research progress and application value in some aspects of forensic medicine, such as the effects of drugs/toxins on the growth and development of sarcosaphagous insects and the qualitative and quantitative analysis of the drugs/toxins in the poisoned body tissue. Related Articles | Metrics

Select

Sudden Death due to Diffuse Alveolar Hemorrhage Induced by Pulmonary Renal Syndrome: Two Case Reports DENG KAI-FEI, SHEN SHAN-SHAN, CHEN YI-JIU, ET AL. 2015, 31(3): 246-248.  DOI: 10.3969/j.issn.1004-5619.2015.03.020 Abstract ( 767 )   Related Articles | Metrics

Select

Fatal Acute Compartment Syndrome Following a Falling Injury: A Case Report WANG TAO, TAO LU-YANG, ZHANG JIAN-HUA, ET AL. 2015, 31(3): 249-251.  DOI: 10.3969/j.issn.1004-5619.2015.03.021 Abstract ( 708 )   Related Articles | Metrics