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28 August 2015, Volume 31 Issue 4 Previous Issue    Next Issue

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Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fatal Hyperthermia WANG ZHI-JUN, SHEN SHAN-SHAN, DENG KAI-FEI, ET AL. 2015, 31(4): 257-261.  DOI: 10.3969/j.issn.1004-5619.2015.04.001 Abstract ( 970 )   Objective To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy （FTIR-MSP） and to provide a new method to diagnose fatal hyperthermia. Methods Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP. Results The absorbances of 3 290, 3 070, 2 850, 1 540 and 1 396 cm-1 significantly increased （P<0.05）, and the ratios of A1 650/A3 290 and A1 650/A1 540 significantly decreased （P<0.05） in group of hyperthermia. Conclusion FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death. Related Articles | Metrics

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Cardiac Ultrastructure and Changes of HSP70 and HIF-1α Expression in Electric Shock Death Rats ZHANG GUO-ZHONG, LI RUI-LI, FENG GUO-WEI, ET AL. 2015, 31(4): 262-265.  DOI: 10.3969/j.issn.1004-5619.2015.04.002 Abstract ( 954 )   Objective To observe cardiac ultrastructure and the expression of heat shock protein 70 （HSP70） and hypoxia inducible factor-1α （HIF-1α） in electric shock death rats and to explore the application of these indexes as the basis of medical identification in electric shock death. Methods Seventy-two SD rats were randomly divided into electric shock death group, postmortem electric shock group and the control group. The changes of myocardial ultrastructure were observed by transmission electron microscope, and the expressions of myocardial HSP70 and HIF-1α were observed by immunohistochemical technology. Results Myocardial myofibril fracture, mitochondrial cristae and membrane dissolution, and disordered arrangement of Z lines and M lines were observed in electric shock rats. HSP70 and HIF-1α were strong positive expressions in the electric shock death group, significantly compared with the control and postmortem electric shock groups （P<0.05）. Conclusion The expressions of HSP70 and HIF-1α were obviously increased in electric shock death group, which may be used as the diagnostic indicator of electric shock death. Related Articles | Metrics

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Changes of HCN4, Cx43 Expression in the Sinoatrial Node of Electric Shock Death CHEN XIAO-FENG, LIANG DONG, HAN QI, ET AL. 2015, 31(4): 266-268.  DOI: 10.3969/j.issn.1004-5619.2015.04.003 Abstract ( 928 )   Objective To investigate the expression of hyperpolarization-activated cyclic nucleotide-gated cation channel 4 （HCN4） and connexin43 （Cx43） in the sinoatrial node of electric shock death. Methods As experimental group, 34 cases of electric shock death who had definite current mark evidence were selected from pathology department of Xuzhou Medical College from 2010 to 2013. As the control group, 20 cases of fatal severe craniocerebral injury in traffic accidents were chosen. The expressions of HCN4 and Cx43 in the sinoatrial node were observed by immunohistochemical technology. Results HCN4 positive cells expressed in the cell membrane and cytoplasm of the sinoatrial node. Cx43 positive cells expressed in the cell membrane and cytoplasm of T cells and myocardial cells. The expression of HCN4 was significantly higher than that of the control group （P<0.05） and the expression of Cx43 was significantly lower than that of the control group （P<0.05）. Conclusion The changes of HCN4 and Cx43 expressions in the sinoatrial node illustrate electric shock death might be related to the abnormalities of cardiac electrophysiology and conduction. Related Articles | Metrics

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Genetic Polymorphisms of 26 Y-STR Loci in Fujian She Nationality and Its Forensic Application BIAN YING-NAN, TE LAI-TI-·SAI-YI-TI, ZHU RU-XIN, ET AL. 2015, 31(4): 269-272.  DOI: 10.3969/j.issn.1004-5619.2015.04.004 Abstract ( 1181 )   Objective To study the forensic application of GoldeneyeTM DNA ID 26Y Kit in the She nationality. Methods Through capillary electrophoresis, the genotype of 26 Y-STR loci were analyzed in 53 unrelated male individuals from Fujian She nationality. The population genetics parameters such as allele frequency and haplotype diversity were calculated. The comparisons among the She nationality and the other nationalities were analyzed. Results A total of 126 alleles were observed on the 26 Y-STR loci of 53 unrelated male individuals. The allele frequencies and GD value ranged from 0.010 1 to 0.886 8 and 0.211 2 to 0.846 2, respectively. The GD value was greater than 0.5 in the 19 loci. A total of 47 haplotypes were observed. Based on RST, multidimensional scaling plot indicated that the genetic relationship among Fujian She nationality and Minnan Han nationality was closest, followed by Southern China Han nationality and Northern China nationality. Conclusion GoldeneyeTM DNA ID 26Y Kit including 26 Y-STR loci has good polymorphism in the She nationality. As an additional system, it has forensic application value in some special cases. Related Articles | Metrics

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Validation of GlobalFiler?誖PCR Amplification Kit and the STR Polymorphism LU ZHI-YONG, XUE LU-YAN, ZHANG QING-XIA, ET AL. 2015, 31(4): 273-176.  DOI: 10.3969/j.issn.1004-5619.2015.04.005 Abstract ( 1157 )   Objective To test the technical parameters of GlobalFiler?誖PCR Amplification Kit for its application to forensic application value and to investigate the genetic polymorphisms. Methods The validation was conducted in sensitivity, mixed samples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The amplification and detection of the genomic DNA from 373 unrelated individuals from Beijing Han nationality were extracted by automation workstation. Results GlobalFiler?誖PCR Amplification Kit was adaptive to some mixed, degraded and inhibited samples. The power of sensitivity and adaptability and peak height balance showed well. The distributions of genotype frequencies for 21 STR loci in the population were all in accordance with Hardy-Weinberg equilibrium （P＞0.05）. The PIC value of the 21 STR loci was among 0.536 to 0.940; the H value was among 0.558 to 0.933; the DP value was among 0.783 to 0.992; the PE value was among 0.243 to 0.874. Conclusion GlobalFiler?誖PCR Amplification Kit is suitable for criminal cases and DNA database in forensic practice. And 21 STR loci in Beijing Han nationality have high polymorphism, which have application value in forensic practice and population genetics. Related Articles | Metrics

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Long Backbone Fracture Caused by Blunt Force: 99 Cases Analysis YU YAN-HE, YANG CHUN-ZHI, WEI QI-CHEN, ET AL. 2015, 31(4): 277-279.  DOI: 10.3969/j.issn.1004-5619.2015.04.006 Abstract ( 961 )   Objective To investigate the main point of long backbone fracture caused by blunt force in forensic clinical identification and to provide a reference for the inspection and appraisal practices of such injury. Methods Ninety-nine cases of adult long backbone fractures were collected from January 2006 to December 2013 in Gutian County of Fujian Province. According to the terms of fracture location, mode of injury, type, the data were summarized. Results In the 99 cases, there were 36 cases caused by hitting, kicking, and falling and 63 cases caused by vehicle collision. The majority of the former was ulna, and those of the latter were tibia and fibula. The types of fracture were transverse one, short oblique one, long oblique one, and spiral one. Conclusion Different types of long backbone fracture, not only causing stress load of fractures as well as structural differences related to each segment. Related Articles | Metrics

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Forensic Investigation of GoldeneyeTM DNA ID 22NC Kit LIN YUAN, QUE TING-ZHI, ZHAO ZHEN-MIN, ET AL. 2015, 31(4): 280-283.  DOI: 10.3969/j.issn.1004-5619.2015.04.007 Abstract ( 1325 )   Objective To investigate the genetic data of 21 autosomal STR included in GoldeneyeTM DNA ID 22NC Kit in Chinese Han nationality and to evaluate the forensic application. Methods By detected 500 unrelated healthy individuals in Chinese Han nationality of East China with GoldeneyeTM DNA ID 22NC Kit, allele frequencies, population genetics parameters and linkage disequilibrium information of the 21 autosomal STR were statistically analyzed. Results In the 21 autosomal STR, no deviations from Hardy-Weinberg equilibrium were detected and all loci were independent form each other. DP values of 21 autosomal STR were all above 0.85, and the combined discrimination power was 1-3.616　5×10-26. Combined mean exclusion chance of this system in duo cases was 1-2.786　81×10-6, in trio cases was 1-8.545　82×10-10. Conclusion Twenty-one autosomal STR included in GoldeneyeTM DNA ID 22NC Kit are highly polymorphic in the Han nationality. Combined with GoldeneyeTM DNA ID 20A Kit, the kit can satisfy the needs for full-sibling testing and facilitate the solution of this kind of case tools. Related Articles | Metrics

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Forensic Validation of the GoldeneyeTM DNA ID 25A Kit SUN YAO-DONG, CAO LI-PING 2015, 31(4): 284-286.  DOI: 10.3969/j.issn.1004-5619.2015.04.008 Abstract ( 1692 )   Objective To test and estimate the forensic application of GoldeneyeTM DNA ID 25A Kit. Methods The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. Results The peak height balance of the different loci was ≥42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. Conclusion GoldeneyeTM DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice. Related Articles | Metrics

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Research Advances in Postmortem Chemistry HAN SHUN-QI, QIN ZHI-QIANG, DENG KAI-FEI, ET AL. 2015, 31(4): 287-292.  DOI: 10.3969/j.issn.1004-5619.2015.04.009 Abstract ( 967 )   Postmortem chemistry is becoming more and more essential in routine forensic pathology and has made considerable progress over the past years. Biochemical analyses of vitreous humor, blood, urine and cerebrospinal fluid may provide important information in determining the cause of death or in elucidating forensic issues. Postmortem chemistry may be essential for the determination of cause of death when morphological methods （diabetes mellitus, alcoholic ketoacidosis and electrolytic disorders） cannot detect the pathophysiological changes involved in the death process. It can also provide many information in other forensic situations, including myocardial ischemia, sepsis, inflammation, infection, anaphylaxis and hormonal disturbances. The most recent relevant research advances on glucose metabolism, liver function, cardiac function, renal function, sepsis, inflammation, infection, anaphylaxis and hormonal aspect are hereby reviewed. Related Articles | Metrics

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Acute Pharyngeal Hemorrhage as a Fatal Complication Arising from Acupotomy with Needle-knife: A Case Report ZHANG LIN, DUAN HUI-JIE, XING JING-JUN, DENG 2015, 31(4): 317-319.  DOI: 10.3969/j.issn.1004-5619.2015.04.018 Abstract ( 867 )   Related Articles | Metrics