Abstract: Distributions and gene frequencies of Gc and Tf phenotypes were examined simultaneously by slab electrophoresis of polyarylamibe in serum of Shanghai China, The obtained allele frequencies are as follows Gc~1=0.6629,Gc~2=0.3371,Tf~c=0.9839, Tf~b=0.0048, Tf~d=0.0113. The discriminat powers (Dp Values) of Gc and Tf phenotypes is 0.612. This method is useful in individual identification and paternity testing in the fields of frensic science, clicical biochemestry、anthropologic genetics and hematology.